ssr_primer
Primer Details for TRINITY_DN12587_c4_g3_i2 |
|---|
| (CTT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACAATATTGACCCGCGCTG | GTGAACGTGTGAAGTGCCAC | Start: 251 |
| Tm | 59.972 | 59.972 | End: 366 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | GGGAGAGAAGGAGGGCAAAC | CTGGAGTTCAGACGTGTGCT | Start: 3376 |
| Tm | 60.035 | 59.967 | End: 3489 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | ACGGAGGCTTCGATCATGTC | GGCTTCATTTCGTCATCGGC | Start: 1574 |
| Tm | 59.897 | 59.972 | End: 1749 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | CACAATATTGACCCGCGCTG | ACGTCATATGTGGCACTGGG | Start: 251 |
| Tm | 59.972 | 60.108 | End: 417 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | ACGGAGGCTTCGATCATGTC | GGCTTTTTGGACGGAAACCC | Start: 1574 |
| Tm | 59.897 | 59.967 | End: 1732 |
| Size | 20 | 20 | 159 |
(A)13gttcaaaaagttggaaattgacattttttaaaacgtttgagagagagggagagag(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACAATATTGACCCGCGCTG | GTGAACGTGTGAAGTGCCAC | Start: 251 |
| Tm | 59.972 | 59.972 | End: 366 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | GGGAGAGAAGGAGGGCAAAC | CTGGAGTTCAGACGTGTGCT | Start: 3376 |
| Tm | 60.035 | 59.967 | End: 3489 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | ACGGAGGCTTCGATCATGTC | GGCTTCATTTCGTCATCGGC | Start: 1574 |
| Tm | 59.897 | 59.972 | End: 1749 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | CACAATATTGACCCGCGCTG | ACGTCATATGTGGCACTGGG | Start: 251 |
| Tm | 59.972 | 60.108 | End: 417 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | ACGGAGGCTTCGATCATGTC | GGCTTTTTGGACGGAAACCC | Start: 1574 |
| Tm | 59.897 | 59.967 | End: 1732 |
| Size | 20 | 20 | 159 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACAATATTGACCCGCGCTG | GTGAACGTGTGAAGTGCCAC | Start: 251 |
| Tm | 59.972 | 59.972 | End: 366 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | GGGAGAGAAGGAGGGCAAAC | CTGGAGTTCAGACGTGTGCT | Start: 3376 |
| Tm | 60.035 | 59.967 | End: 3489 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | ACGGAGGCTTCGATCATGTC | GGCTTCATTTCGTCATCGGC | Start: 1574 |
| Tm | 59.897 | 59.972 | End: 1749 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | CACAATATTGACCCGCGCTG | ACGTCATATGTGGCACTGGG | Start: 251 |
| Tm | 59.972 | 60.108 | End: 417 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | ACGGAGGCTTCGATCATGTC | GGCTTTTTGGACGGAAACCC | Start: 1574 |
| Tm | 59.897 | 59.967 | End: 1732 |
| Size | 20 | 20 | 159 |
(AAGA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACAATATTGACCCGCGCTG | GTGAACGTGTGAAGTGCCAC | Start: 251 |
| Tm | 59.972 | 59.972 | End: 366 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | GGGAGAGAAGGAGGGCAAAC | CTGGAGTTCAGACGTGTGCT | Start: 3376 |
| Tm | 60.035 | 59.967 | End: 3489 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | ACGGAGGCTTCGATCATGTC | GGCTTCATTTCGTCATCGGC | Start: 1574 |
| Tm | 59.897 | 59.972 | End: 1749 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | CACAATATTGACCCGCGCTG | ACGTCATATGTGGCACTGGG | Start: 251 |
| Tm | 59.972 | 60.108 | End: 417 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | ACGGAGGCTTCGATCATGTC | GGCTTTTTGGACGGAAACCC | Start: 1574 |
| Tm | 59.897 | 59.967 | End: 1732 |
| Size | 20 | 20 | 159 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACAATATTGACCCGCGCTG | GTGAACGTGTGAAGTGCCAC | Start: 251 |
| Tm | 59.972 | 59.972 | End: 366 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | GGGAGAGAAGGAGGGCAAAC | CTGGAGTTCAGACGTGTGCT | Start: 3376 |
| Tm | 60.035 | 59.967 | End: 3489 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | ACGGAGGCTTCGATCATGTC | GGCTTCATTTCGTCATCGGC | Start: 1574 |
| Tm | 59.897 | 59.972 | End: 1749 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | CACAATATTGACCCGCGCTG | ACGTCATATGTGGCACTGGG | Start: 251 |
| Tm | 59.972 | 60.108 | End: 417 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | ACGGAGGCTTCGATCATGTC | GGCTTTTTGGACGGAAACCC | Start: 1574 |
| Tm | 59.897 | 59.967 | End: 1732 |
| Size | 20 | 20 | 159 |
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