ssr_primer
Primer Details for TRINITY_DN12586_c1_g1_i6 |
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| (AAG)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCTTCCCTTCACCGCAAT | TTGGGGAGGATACAAACGGC | Start: 1143 |
| Tm | 60.035 | 60.035 | End: 1332 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
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| Sequence | GGTTGTCCTCTCCACCAAGG | GCCAGTGTCCCGATTCAGAA | Start: 521 |
| Tm | 59.963 | 60.036 | End: 673 |
| Size | 20 | 20 | 153 |
| Primer 3 details |
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| Sequence | TCCTCCATCTTCCTCCCCAG | CCACTTGGGCACAGGTTAGT | Start: 2737 |
| Tm | 60.031 | 59.89 | End: 2858 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
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| Sequence | CTCCTCCATCTTCCTCCCCA | CCACTTGGGCACAGGTTAGT | Start: 2736 |
| Tm | 60.031 | 59.89 | End: 2858 |
| Size | 20 | 20 | 123 |
| Primer 5 details |
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| Sequence | CCTCTTCCCTTCACCGCAAT | AGGCGACAACACAAGGTTCA | Start: 1143 |
| Tm | 60.035 | 60.107 | End: 1361 |
| Size | 20 | 20 | 219 |
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