ssr_primer
Primer Details for TRINITY_DN12586_c0_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCCAAAGTGAACACACTG | GCGAAATGGGTGGGTGAGTA | Start: 213 |
| Tm | 59.971 | 60.036 | End: 377 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
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| Sequence | GCCCTTGCTTCATCTTCCCT | GCGAAATGGGTGGGTGAGTA | Start: 253 |
| Tm | 60.034 | 60.036 | End: 377 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
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| Sequence | TACTCACCCACCCATTTCGC | TTCGACCGTGGATTTGCTGA | Start: 358 |
| Tm | 60.036 | 59.966 | End: 598 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
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| Sequence | TGGTTGAGGATGCTGAGCTG | ATCAACCAGCCCGTTATGCA | Start: 1283 |
| Tm | 60.036 | 60.035 | End: 1383 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
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| Sequence | TGCATAACGGGCTGGTTGAT | AGGTCTCTCTTCGCTGTTGC | Start: 1364 |
| Tm | 60.035 | 60.038 | End: 1634 |
| Size | 20 | 20 | 271 |
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