Primer Details for TRINITY_DN12578_c0_g1_i2 | |||
|---|---|---|---|
| (A)15gttaaaaagtaaaatgtaatgcgggaactggacttgaaagtattatttaacatgggcaataataagtg(AT)7: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGGGAACTGGACTTGAAA | TCATGTCTTAACAAGTTGGAAGGA | Start: 205 |
| Tm | 59.818 | 58.064 | End: 306 |
| Size | 20 | 24 | 102 |
| Primer 2 details | |||
| Sequence | TGCGGGAACTGGACTTGAAA | TGTTCATGTCTTAACAAGTTGGAAGG | Start: 205 |
| Tm | 59.818 | 59.961 | End: 309 |
| Size | 20 | 26 | 105 |
| Primer 3 details | |||
| Sequence | ATGCGGGAACTGGACTTGAA | TCATGTCTTAACAAGTTGGAAGGA | Start: 204 |
| Tm | 59.6 | 58.064 | End: 306 |
| Size | 20 | 24 | 103 |
| Primer 4 details | |||
| Sequence | AATGCGGGAACTGGACTTGA | TCATGTCTTAACAAGTTGGAAGGA | Start: 203 |
| Tm | 59.6 | 58.064 | End: 306 |
| Size | 20 | 24 | 104 |
| Primer 5 details | |||
| Sequence | ATGCGGGAACTGGACTTGAA | TGTTCATGTCTTAACAAGTTGGAAGG | Start: 204 |
| Tm | 59.6 | 59.961 | End: 309 |
| Size | 20 | 26 | 106 |
