ssr_primer
Primer Details for TRINITY_DN12569_c0_g3_i6 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCCTCAATTTCCGCCTCT | GCTCCCAGCCATTAACACCT | Start: 157 |
| Tm | 59.962 | 60.034 | End: 435 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | TTGCCTCAATTTCCGCCTCT | AGCTCCCAGCCATTAACACC | Start: 157 |
| Tm | 59.962 | 60.034 | End: 436 |
| Size | 20 | 20 | 280 |
| Primer 3 details |
|---|
| Sequence | CCAGGTCCAGGGTTGGTTTT | TCCTAATCGAGGGCCAAGGA | Start: 1694 |
| Tm | 60.106 | 60.032 | End: 1882 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | GGGGAGGAAGATGGTTTCGG | TCCTAATCGAGGGCCAAGGA | Start: 1630 |
| Tm | 60.107 | 60.032 | End: 1882 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | TCTGAACAATTCCCTCGCCC | TGGTCAAAAGCTGCAAAGGC | Start: 2722 |
| Tm | 60.035 | 59.895 | End: 2962 |
| Size | 20 | 20 | 241 |
(A)13cagaagtaggactccttaaggttatacaagtgcc(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCCTCAATTTCCGCCTCT | GCTCCCAGCCATTAACACCT | Start: 157 |
| Tm | 59.962 | 60.034 | End: 435 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | TTGCCTCAATTTCCGCCTCT | AGCTCCCAGCCATTAACACC | Start: 157 |
| Tm | 59.962 | 60.034 | End: 436 |
| Size | 20 | 20 | 280 |
| Primer 3 details |
|---|
| Sequence | CCAGGTCCAGGGTTGGTTTT | TCCTAATCGAGGGCCAAGGA | Start: 1694 |
| Tm | 60.106 | 60.032 | End: 1882 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | GGGGAGGAAGATGGTTTCGG | TCCTAATCGAGGGCCAAGGA | Start: 1630 |
| Tm | 60.107 | 60.032 | End: 1882 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | TCTGAACAATTCCCTCGCCC | TGGTCAAAAGCTGCAAAGGC | Start: 2722 |
| Tm | 60.035 | 59.895 | End: 2962 |
| Size | 20 | 20 | 241 |
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