ssr_primer
Primer Details for TRINITY_DN12562_c1_g4_i4 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCGCTGACCTTCATTGTCA | TAAACTCCGAACCGCCGAAA | Start: 8177 |
| Tm | 59.965 | 59.967 | End: 8396 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
|---|
| Sequence | CCTCAGACTGGACCAACGTC | TGAAACCAACAAGGGGGCTT | Start: 7564 |
| Tm | 60.038 | 60.032 | End: 7775 |
| Size | 20 | 20 | 212 |
| Primer 3 details |
|---|
| Sequence | CCTCAGACTGGACCAACGTC | GCTGCTGAAACCAACAAGGG | Start: 7564 |
| Tm | 60.038 | 59.967 | End: 7780 |
| Size | 20 | 20 | 217 |
| Primer 4 details |
|---|
| Sequence | TTTCAATGGCGAGGGAACGA | TGACAATGAAGGTCAGCGCT | Start: 8062 |
| Tm | 59.964 | 59.965 | End: 8196 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | AGGATGCGTGAGGTGAACAG | CTAAAGGTGGCAAGGGGAGG | Start: 2984 |
| Tm | 60.037 | 60.034 | End: 3117 |
| Size | 20 | 20 | 134 |
(TCT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCGCTGACCTTCATTGTCA | TAAACTCCGAACCGCCGAAA | Start: 8177 |
| Tm | 59.965 | 59.967 | End: 8396 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
|---|
| Sequence | CCTCAGACTGGACCAACGTC | TGAAACCAACAAGGGGGCTT | Start: 7564 |
| Tm | 60.038 | 60.032 | End: 7775 |
| Size | 20 | 20 | 212 |
| Primer 3 details |
|---|
| Sequence | CCTCAGACTGGACCAACGTC | GCTGCTGAAACCAACAAGGG | Start: 7564 |
| Tm | 60.038 | 59.967 | End: 7780 |
| Size | 20 | 20 | 217 |
| Primer 4 details |
|---|
| Sequence | TTTCAATGGCGAGGGAACGA | TGACAATGAAGGTCAGCGCT | Start: 8062 |
| Tm | 59.964 | 59.965 | End: 8196 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | AGGATGCGTGAGGTGAACAG | CTAAAGGTGGCAAGGGGAGG | Start: 2984 |
| Tm | 60.037 | 60.034 | End: 3117 |
| Size | 20 | 20 | 134 |
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