ssr_primer
Primer Details for TRINITY_DN12562_c1_g3_i2 |
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| (TG)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGATACTCGTCGTCCATT | ATTCCAAACAGTCGCCTCGT | Start: 5967 |
| Tm | 59.97 | 59.965 | End: 6184 |
| Size | 20 | 20 | 218 |
| Primer 2 details |
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| Sequence | GCCGATACTCGTCGTCCATT | TTTGAGGCGCATGATCAGGT | Start: 5967 |
| Tm | 59.97 | 60.035 | End: 6120 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
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| Sequence | CTCGTCCGGTTTGCACTTTG | TATGGCCAAGATGTTGCGGT | Start: 4425 |
| Tm | 60.041 | 60.035 | End: 4637 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
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| Sequence | CGTCCGGTTTGCACTTTGTT | TATGGCCAAGATGTTGCGGT | Start: 4427 |
| Tm | 59.9 | 60.035 | End: 4637 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
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| Sequence | AGCTCATGACCTTCACCGTG | GGTGTTCGATGAAATGCCCG | Start: 5644 |
| Tm | 60.037 | 59.901 | End: 5772 |
| Size | 20 | 20 | 129 |
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