ssr_primer
Primer Details for TRINITY_DN12558_c1_g2_i17 |
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| (CTC)8gaatcctcttcacacaaaagctccacttgtttctcttcaaatccgccattttctcttcctctgtctgcg(TC)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGCTCCATGAGGATCTCA | ATGGAGTCATTGCGAGCCAA | Start: 5096 |
| Tm | 60.031 | 60.035 | End: 5224 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
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| Sequence | TTGGCTCGCAATGACTCCAT | TCCCATTTTCCGGACTTGGG | Start: 5205 |
| Tm | 60.035 | 59.961 | End: 5431 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
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| Sequence | GCACCACGAACGTTCAAGTC | ATGGAGTCATTGCGAGCCAA | Start: 4998 |
| Tm | 60.042 | 60.035 | End: 5224 |
| Size | 20 | 20 | 227 |
| Primer 4 details |
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| Sequence | GCACCACGAACGTTCAAGTC | CAGATCGCCCATCTCCATCC | Start: 4998 |
| Tm | 60.042 | 60.037 | End: 5169 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
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| Sequence | TGGGCTCCATGAGGATCTCA | TGTTGTTGAAGGCAGAGGCA | Start: 5096 |
| Tm | 60.031 | 60.107 | End: 5342 |
| Size | 20 | 20 | 247 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGCTCCATGAGGATCTCA | ATGGAGTCATTGCGAGCCAA | Start: 5096 |
| Tm | 60.031 | 60.035 | End: 5224 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | TTGGCTCGCAATGACTCCAT | TCCCATTTTCCGGACTTGGG | Start: 5205 |
| Tm | 60.035 | 59.961 | End: 5431 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
|---|
| Sequence | GCACCACGAACGTTCAAGTC | ATGGAGTCATTGCGAGCCAA | Start: 4998 |
| Tm | 60.042 | 60.035 | End: 5224 |
| Size | 20 | 20 | 227 |
| Primer 4 details |
|---|
| Sequence | GCACCACGAACGTTCAAGTC | CAGATCGCCCATCTCCATCC | Start: 4998 |
| Tm | 60.042 | 60.037 | End: 5169 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
|---|
| Sequence | TGGGCTCCATGAGGATCTCA | TGTTGTTGAAGGCAGAGGCA | Start: 5096 |
| Tm | 60.031 | 60.107 | End: 5342 |
| Size | 20 | 20 | 247 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGCTCCATGAGGATCTCA | ATGGAGTCATTGCGAGCCAA | Start: 5096 |
| Tm | 60.031 | 60.035 | End: 5224 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | TTGGCTCGCAATGACTCCAT | TCCCATTTTCCGGACTTGGG | Start: 5205 |
| Tm | 60.035 | 59.961 | End: 5431 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
|---|
| Sequence | GCACCACGAACGTTCAAGTC | ATGGAGTCATTGCGAGCCAA | Start: 4998 |
| Tm | 60.042 | 60.035 | End: 5224 |
| Size | 20 | 20 | 227 |
| Primer 4 details |
|---|
| Sequence | GCACCACGAACGTTCAAGTC | CAGATCGCCCATCTCCATCC | Start: 4998 |
| Tm | 60.042 | 60.037 | End: 5169 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
|---|
| Sequence | TGGGCTCCATGAGGATCTCA | TGTTGTTGAAGGCAGAGGCA | Start: 5096 |
| Tm | 60.031 | 60.107 | End: 5342 |
| Size | 20 | 20 | 247 |
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