ssr_primer
Primer Details for TRINITY_DN12556_c4_g1_i1 |
|---|
| (A)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGCCAGTGCACAAGAACC | ATTCTTCTTCCGAGCCCTGC | Start: 136 |
| Tm | 59.969 | 60.107 | End: 393 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | CAAGCCAGTGCACAAGAACC | CCCTGCCCTCCTCTTTCTTC | Start: 136 |
| Tm | 59.969 | 59.746 | End: 379 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
|---|
| Sequence | GCAGGGCTCGGAAGAAGAAT | TCCAGTGTGCGTTGAAGGAA | Start: 374 |
| Tm | 60.107 | 59.821 | End: 536 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | GCCAGTGCACAAGAACCAAC | ATTCTTCTTCCGAGCCCTGC | Start: 139 |
| Tm | 60.249 | 60.107 | End: 393 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | GCAGGGCTCGGAAGAAGAAT | CTCCAGTGTGCGTTGAAGGA | Start: 374 |
| Tm | 60.107 | 60.25 | End: 537 |
| Size | 20 | 20 | 164 |
(AAT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGCCAGTGCACAAGAACC | ATTCTTCTTCCGAGCCCTGC | Start: 136 |
| Tm | 59.969 | 60.107 | End: 393 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | CAAGCCAGTGCACAAGAACC | CCCTGCCCTCCTCTTTCTTC | Start: 136 |
| Tm | 59.969 | 59.746 | End: 379 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
|---|
| Sequence | GCAGGGCTCGGAAGAAGAAT | TCCAGTGTGCGTTGAAGGAA | Start: 374 |
| Tm | 60.107 | 59.821 | End: 536 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | GCCAGTGCACAAGAACCAAC | ATTCTTCTTCCGAGCCCTGC | Start: 139 |
| Tm | 60.249 | 60.107 | End: 393 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | GCAGGGCTCGGAAGAAGAAT | CTCCAGTGTGCGTTGAAGGA | Start: 374 |
| Tm | 60.107 | 60.25 | End: 537 |
| Size | 20 | 20 | 164 |
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