ssr_primer
Primer Details for TRINITY_DN12556_c3_g5_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAACACCTTTGCCCCTGGA | TCAAGGTCCTGCACTGGTTG | Start: 166 |
| Tm | 60.032 | 60.179 | End: 413 |
| Size | 20 | 20 | 248 |
| Primer 2 details |
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| Sequence | AAAACACCTTTGCCCCTGGA | TTTGGGACTGCTCAGCTGTT | Start: 166 |
| Tm | 60.032 | 59.817 | End: 299 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
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| Sequence | CAACCAGTGCAGGACCTTGA | ACCTCGGTGAATTTGCCCAT | Start: 394 |
| Tm | 60.179 | 59.96 | End: 497 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
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| Sequence | AACAGCTGAGCAGTCCCAAA | ACCTCGGTGAATTTGCCCAT | Start: 280 |
| Tm | 59.817 | 59.96 | End: 497 |
| Size | 20 | 20 | 218 |
| Primer 5 details |
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| Sequence | GAGCAATTCCACGGGGATCA | TCAAGGTCCTGCACTGGTTG | Start: 208 |
| Tm | 60.107 | 60.179 | End: 413 |
| Size | 20 | 20 | 206 |
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