Primer Details for TRINITY_DN12534_c4_g1_i1 | |||
|---|---|---|---|
| (AT)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGCTATCGTTGATAATAGGCA | TCAGGTATGGTCAAAATTGCACT | Start: 12 |
| Tm | 57.223 | 58.594 | End: 215 |
| Size | 22 | 23 | 204 |
| Primer 2 details | |||
| Sequence | CGCTATCGTTGATAATAGGCAAGT | TCAGGTATGGTCAAAATTGCACT | Start: 13 |
| Tm | 59.076 | 58.594 | End: 215 |
| Size | 24 | 23 | 203 |
| Primer 3 details | |||
| Sequence | CGCTATCGTTGATAATAGGCAAGTC | TCAGGTATGGTCAAAATTGCACT | Start: 13 |
| Tm | 59.939 | 58.594 | End: 215 |
| Size | 25 | 23 | 203 |
| Primer 4 details | |||
| Sequence | TCGCTATCGTTGATAATAGGCAA | TCAGGTATGGTCAAAATTGCACT | Start: 12 |
| Tm | 57.871 | 58.594 | End: 215 |
| Size | 23 | 23 | 204 |
| Primer 5 details | |||
| Sequence | TCGCTATCGTTGATAATAGGCA | TCAGGTATGGTCAAAATTGCAC | Start: 12 |
| Tm | 57.223 | 57.218 | End: 215 |
| Size | 22 | 22 | 204 |
