Primer Details for TRINITY_DN12528_c6_g1_i1 | |||
|---|---|---|---|
| (A)17: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTTGATTGGAGGTGGTTGT | TCGAACCAAATTTGTGGAATGT | Start: 99 |
| Tm | 59.501 | 57.072 | End: 269 |
| Size | 21 | 22 | 171 |
| Primer 2 details | |||
| Sequence | AGGTTGATTGGAGGTGGTTGT | TCGAACCAAATTTGTGGAATGTT | Start: 99 |
| Tm | 59.501 | 57.73 | End: 269 |
| Size | 21 | 23 | 171 |
| Primer 3 details | |||
| Sequence | AGGTTGATTGGAGGTGGTTGT | TTCGAACCAAATTTGTGGAATGT | Start: 99 |
| Tm | 59.501 | 57.73 | End: 270 |
| Size | 21 | 23 | 172 |
| Primer 4 details | |||
| Sequence | GGTTGATTGGAGGTGGTTGT | TCGAACCAAATTTGTGGAATGT | Start: 100 |
| Tm | 58.005 | 57.072 | End: 269 |
| Size | 20 | 22 | 170 |
| Primer 5 details | |||
| Sequence | AGGTTGATTGGAGGTGGTTGT | TGGCTTTGTTTTGCAATAGATCTCT | Start: 99 |
| Tm | 59.501 | 59.522 | End: 233 |
| Size | 21 | 25 | 135 |
