ssr_primer
Primer Details for TRINITY_DN12525_c1_g5_i6 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTGTAACAGCCGCCGTTG | GCAGAGCTGGGAATGTCCTT | Start: 71 |
| Tm | 59.97 | 60.035 | End: 257 |
| Size | 20 | 20 | 187 |
| Primer 2 details |
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| Sequence | TTTTGTAACAGCCGCCGTTG | GGGACTTGGAAGCGACTGAA | Start: 71 |
| Tm | 59.97 | 59.965 | End: 170 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
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| Sequence | TTGCTTTTGTAACAGCCGCC | GCAGAGCTGGGAATGTCCTT | Start: 67 |
| Tm | 59.969 | 60.035 | End: 257 |
| Size | 20 | 20 | 191 |
| Primer 4 details |
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| Sequence | TTGCTTTTGTAACAGCCGCC | GGGACTTGGAAGCGACTGAA | Start: 67 |
| Tm | 59.969 | 59.965 | End: 170 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
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| Sequence | TTGCTTTTGTAACAGCCGCC | CTTGGAAGCGACTGAAGGGT | Start: 67 |
| Tm | 59.969 | 59.965 | End: 166 |
| Size | 20 | 20 | 100 |
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