ssr_primer
Primer Details for TRINITY_DN12525_c1_g3_i1 |
|---|
| (AT)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCGGCTCCTCTCGATCGTA | TGGCTATGGAGGGTTTGCAG | Start: 391 |
| Tm | 59.965 | 60.034 | End: 603 |
| Size | 20 | 20 | 213 |
| Primer 2 details |
|---|
| Sequence | TCCCAAACGCGCTACAGAAT | TACGATCGAGAGGAGCCGAT | Start: 292 |
| Tm | 60.037 | 59.965 | End: 410 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | AACGCGCTACAGAATCCACA | TACGATCGAGAGGAGCCGAT | Start: 297 |
| Tm | 60.038 | 59.965 | End: 410 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | ATCGGCTCCTCTCGATCGTA | GGCAAGGCTCATCGGGATTA | Start: 391 |
| Tm | 59.965 | 59.891 | End: 561 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | TCCCAAACGCGCTACAGAAT | GGCAAGGCTCATCGGGATTA | Start: 292 |
| Tm | 60.037 | 59.891 | End: 561 |
| Size | 20 | 20 | 270 |
(GCC)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCGGCTCCTCTCGATCGTA | TGGCTATGGAGGGTTTGCAG | Start: 391 |
| Tm | 59.965 | 60.034 | End: 603 |
| Size | 20 | 20 | 213 |
| Primer 2 details |
|---|
| Sequence | TCCCAAACGCGCTACAGAAT | TACGATCGAGAGGAGCCGAT | Start: 292 |
| Tm | 60.037 | 59.965 | End: 410 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | AACGCGCTACAGAATCCACA | TACGATCGAGAGGAGCCGAT | Start: 297 |
| Tm | 60.038 | 59.965 | End: 410 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | ATCGGCTCCTCTCGATCGTA | GGCAAGGCTCATCGGGATTA | Start: 391 |
| Tm | 59.965 | 59.891 | End: 561 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | TCCCAAACGCGCTACAGAAT | GGCAAGGCTCATCGGGATTA | Start: 292 |
| Tm | 60.037 | 59.891 | End: 561 |
| Size | 20 | 20 | 270 |
(AG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCGGCTCCTCTCGATCGTA | TGGCTATGGAGGGTTTGCAG | Start: 391 |
| Tm | 59.965 | 60.034 | End: 603 |
| Size | 20 | 20 | 213 |
| Primer 2 details |
|---|
| Sequence | TCCCAAACGCGCTACAGAAT | TACGATCGAGAGGAGCCGAT | Start: 292 |
| Tm | 60.037 | 59.965 | End: 410 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | AACGCGCTACAGAATCCACA | TACGATCGAGAGGAGCCGAT | Start: 297 |
| Tm | 60.038 | 59.965 | End: 410 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | ATCGGCTCCTCTCGATCGTA | GGCAAGGCTCATCGGGATTA | Start: 391 |
| Tm | 59.965 | 59.891 | End: 561 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | TCCCAAACGCGCTACAGAAT | GGCAAGGCTCATCGGGATTA | Start: 292 |
| Tm | 60.037 | 59.891 | End: 561 |
| Size | 20 | 20 | 270 |
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