Primer Details for TRINITY_DN12524_c0_g1_i1 |
|---|
| (TA)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAATTACAAACTCCTGGGTTTTAGT | TTTTCTTGACCCCTCGCCC | Start: 4 |
| Tm | 57.092 | 59.926 | End: 157 |
| Size | 25 | 19 | 154 |
| Primer 2 details |
|---|
| Sequence | AAATTACAAACTCCTGGGTTTTAGT | GTTTTCTTGACCCCTCGCCC | Start: 4 |
| Tm | 57.092 | 61.242 | End: 158 |
| Size | 25 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | AAATTACAAACTCCTGGGTTTTAGT | GGTTTTCTTGACCCCTCGCC | Start: 4 |
| Tm | 57.092 | 61.242 | End: 159 |
| Size | 25 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | AAATTACAAACTCCTGGGTTTTAGT | TTCTTGACCCCTCGCCCTTT | Start: 4 |
| Tm | 57.092 | 61.424 | End: 155 |
| Size | 25 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | AAATTACAAACTCCTGGGTTTTAGT | TTTCTTGACCCCTCGCCCTT | Start: 4 |
| Tm | 57.092 | 61.424 | End: 156 |
| Size | 25 | 20 | 153 |
