Primer Details for TRINITY_DN12523_c6_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCATACCTAGGTCCAAACC | CACTCTCTTTCCACCTAAGAAAAGA | Start: 7 |
| Tm | 58.515 | 58.474 | End: 209 |
| Size | 21 | 25 | 203 |
| Primer 2 details |
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| Sequence | TCCCATACCTAGGTCCAAACC | ACTCTCTTTCCACCTAAGAAAAGA | Start: 7 |
| Tm | 58.515 | 57.251 | End: 208 |
| Size | 21 | 24 | 202 |
| Primer 3 details |
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| Sequence | TCCCATACCTAGGTCCAAACC | CACTCTCTTTCCACCTAAGAAAAG | Start: 7 |
| Tm | 58.515 | 57.207 | End: 209 |
| Size | 21 | 24 | 203 |
| Primer 4 details |
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| Sequence | TCCCATACCTAGGTCCAAACC | CACTCTCTTTCCACCTAAGAAAAGAA | Start: 7 |
| Tm | 58.515 | 59.007 | End: 209 |
| Size | 21 | 26 | 203 |
| Primer 5 details |
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| Sequence | ATCCCATACCTAGGTCCAAACC | CACTCTCTTTCCACCTAAGAAAAGA | Start: 6 |
| Tm | 58.946 | 58.474 | End: 209 |
| Size | 22 | 25 | 204 |
