ssr_primer
Primer Details for TRINITY_DN12523_c2_g1_i5 |
|---|
| (A)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCGAAACTCCGACGCAAA | TAACCGAATGCCCTCCCCTA | Start: 74 |
| Tm | 59.969 | 60.031 | End: 300 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
|---|
| Sequence | TTCTCGAAACTCCGACGCAA | TAACCGAATGCCCTCCCCTA | Start: 73 |
| Tm | 59.969 | 60.031 | End: 300 |
| Size | 20 | 20 | 228 |
| Primer 3 details |
|---|
| Sequence | TGCCGGTACCTTCTCGAAAC | TAACCGAATGCCCTCCCCTA | Start: 63 |
| Tm | 60.038 | 60.031 | End: 300 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | GAAAGATTCTCGCCGGTTGC | TAACCGAATGCCCTCCCCTA | Start: 40 |
| Tm | 59.903 | 60.031 | End: 300 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TAGGGGAGGGCATTCGGTTA | ATGAGGCTGCCCTGCTTTAG | Start: 281 |
| Tm | 60.031 | 60.107 | End: 485 |
| Size | 20 | 20 | 205 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCGAAACTCCGACGCAAA | TAACCGAATGCCCTCCCCTA | Start: 74 |
| Tm | 59.969 | 60.031 | End: 300 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
|---|
| Sequence | TTCTCGAAACTCCGACGCAA | TAACCGAATGCCCTCCCCTA | Start: 73 |
| Tm | 59.969 | 60.031 | End: 300 |
| Size | 20 | 20 | 228 |
| Primer 3 details |
|---|
| Sequence | TGCCGGTACCTTCTCGAAAC | TAACCGAATGCCCTCCCCTA | Start: 63 |
| Tm | 60.038 | 60.031 | End: 300 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | GAAAGATTCTCGCCGGTTGC | TAACCGAATGCCCTCCCCTA | Start: 40 |
| Tm | 59.903 | 60.031 | End: 300 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TAGGGGAGGGCATTCGGTTA | ATGAGGCTGCCCTGCTTTAG | Start: 281 |
| Tm | 60.031 | 60.107 | End: 485 |
| Size | 20 | 20 | 205 |
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