ssr_primer
Primer Details for TRINITY_DN12523_c1_g1_i2 |
|---|
| (TC)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATTTCGAATTGGAGGCGCC | GAACCACAACCTGAGGCGTA | Start: 338 |
| Tm | 59.971 | 59.966 | End: 503 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
|---|
| Sequence | CATCCGATCCGATTCCCCTG | GGCGCCTCCAATTCGAAATC | Start: 86 |
| Tm | 60.037 | 59.971 | End: 357 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | TGGCACCGTCACTTTCTCTC | TCAACCTCCAAGGACGAAGC | Start: 826 |
| Tm | 59.966 | 59.965 | End: 1003 |
| Size | 20 | 20 | 178 |
| Primer 4 details |
|---|
| Sequence | TCTGGCACCGTCACTTTCTC | TCAACCTCCAAGGACGAAGC | Start: 824 |
| Tm | 59.966 | 59.965 | End: 1003 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
|---|
| Sequence | TGGCACCGTCACTTTCTCTC | CCTCCAAGGACGAAGCCAAT | Start: 826 |
| Tm | 59.966 | 60.035 | End: 999 |
| Size | 20 | 20 | 174 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATTTCGAATTGGAGGCGCC | GAACCACAACCTGAGGCGTA | Start: 338 |
| Tm | 59.971 | 59.966 | End: 503 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
|---|
| Sequence | CATCCGATCCGATTCCCCTG | GGCGCCTCCAATTCGAAATC | Start: 86 |
| Tm | 60.037 | 59.971 | End: 357 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | TGGCACCGTCACTTTCTCTC | TCAACCTCCAAGGACGAAGC | Start: 826 |
| Tm | 59.966 | 59.965 | End: 1003 |
| Size | 20 | 20 | 178 |
| Primer 4 details |
|---|
| Sequence | TCTGGCACCGTCACTTTCTC | TCAACCTCCAAGGACGAAGC | Start: 824 |
| Tm | 59.966 | 59.965 | End: 1003 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
|---|
| Sequence | TGGCACCGTCACTTTCTCTC | CCTCCAAGGACGAAGCCAAT | Start: 826 |
| Tm | 59.966 | 60.035 | End: 999 |
| Size | 20 | 20 | 174 |
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