ssr_primer
Primer Details for TRINITY_DN12522_c4_g2_i4 |
|---|
| (T)10ctttgaacattagtctcactaactcgtttccgtttcagg(A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGCGCAATCCTTTTGTGG | TCCCGATGAACAACCCCAAG | Start: 3827 |
| Tm | 59.968 | 59.962 | End: 4033 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
|---|
| Sequence | GGGCATTTGGTCGGGTTTTC | CCACAAAAGGATTGCGCACA | Start: 3691 |
| Tm | 60.038 | 59.968 | End: 3846 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
|---|
| Sequence | GTGCGCAATCCTTTTGTGGT | TCCCGATGAACAACCCCAAG | Start: 3828 |
| Tm | 59.968 | 59.962 | End: 4033 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
|---|
| Sequence | TGTGCGCAATCCTTTTGTGG | AAGTCCCGATGAACAACCCC | Start: 3827 |
| Tm | 59.968 | 59.962 | End: 4036 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | GGGCATTTGGTCGGGTTTTC | ACCACAAAAGGATTGCGCAC | Start: 3691 |
| Tm | 60.038 | 59.968 | End: 3847 |
| Size | 20 | 20 | 157 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGCGCAATCCTTTTGTGG | TCCCGATGAACAACCCCAAG | Start: 3827 |
| Tm | 59.968 | 59.962 | End: 4033 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
|---|
| Sequence | GGGCATTTGGTCGGGTTTTC | CCACAAAAGGATTGCGCACA | Start: 3691 |
| Tm | 60.038 | 59.968 | End: 3846 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
|---|
| Sequence | GTGCGCAATCCTTTTGTGGT | TCCCGATGAACAACCCCAAG | Start: 3828 |
| Tm | 59.968 | 59.962 | End: 4033 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
|---|
| Sequence | TGTGCGCAATCCTTTTGTGG | AAGTCCCGATGAACAACCCC | Start: 3827 |
| Tm | 59.968 | 59.962 | End: 4036 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | GGGCATTTGGTCGGGTTTTC | ACCACAAAAGGATTGCGCAC | Start: 3691 |
| Tm | 60.038 | 59.968 | End: 3847 |
| Size | 20 | 20 | 157 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGCGCAATCCTTTTGTGG | TCCCGATGAACAACCCCAAG | Start: 3827 |
| Tm | 59.968 | 59.962 | End: 4033 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
|---|
| Sequence | GGGCATTTGGTCGGGTTTTC | CCACAAAAGGATTGCGCACA | Start: 3691 |
| Tm | 60.038 | 59.968 | End: 3846 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
|---|
| Sequence | GTGCGCAATCCTTTTGTGGT | TCCCGATGAACAACCCCAAG | Start: 3828 |
| Tm | 59.968 | 59.962 | End: 4033 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
|---|
| Sequence | TGTGCGCAATCCTTTTGTGG | AAGTCCCGATGAACAACCCC | Start: 3827 |
| Tm | 59.968 | 59.962 | End: 4036 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | GGGCATTTGGTCGGGTTTTC | ACCACAAAAGGATTGCGCAC | Start: 3691 |
| Tm | 60.038 | 59.968 | End: 3847 |
| Size | 20 | 20 | 157 |
(AT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGCGCAATCCTTTTGTGG | TCCCGATGAACAACCCCAAG | Start: 3827 |
| Tm | 59.968 | 59.962 | End: 4033 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
|---|
| Sequence | GGGCATTTGGTCGGGTTTTC | CCACAAAAGGATTGCGCACA | Start: 3691 |
| Tm | 60.038 | 59.968 | End: 3846 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
|---|
| Sequence | GTGCGCAATCCTTTTGTGGT | TCCCGATGAACAACCCCAAG | Start: 3828 |
| Tm | 59.968 | 59.962 | End: 4033 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
|---|
| Sequence | TGTGCGCAATCCTTTTGTGG | AAGTCCCGATGAACAACCCC | Start: 3827 |
| Tm | 59.968 | 59.962 | End: 4036 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | GGGCATTTGGTCGGGTTTTC | ACCACAAAAGGATTGCGCAC | Start: 3691 |
| Tm | 60.038 | 59.968 | End: 3847 |
| Size | 20 | 20 | 157 |
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