ssr_primer
Primer Details for TRINITY_DN12520_c0_g1_i21 |
|---|
| (T)31: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACTTGGGGATCGTCGTTT | AGGAGGATGCCACTAGAGCA | Start: 818 |
| Tm | 60.037 | 60.032 | End: 1083 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | TGCTCTAGTGGCATCCTCCT | CTAGTGGAGGTGGAGGTGGA | Start: 1064 |
| Tm | 60.032 | 59.959 | End: 1281 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | TCGAGTTCCTGAAACCGTCG | AATACAACCCACCTCCAGCG | Start: 4608 |
| Tm | 60.04 | 60.035 | End: 4851 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | TTTGATGCGCAACCAGAAGC | CGACGGTTTCAGGAACTCGA | Start: 4497 |
| Tm | 60.039 | 60.04 | End: 4627 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | TCCACCTCCACCTCCACTAG | CCGCAGACTACGTGTTGAGT | Start: 1262 |
| Tm | 59.959 | 60.039 | End: 1445 |
| Size | 20 | 20 | 184 |
(TC)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACTTGGGGATCGTCGTTT | AGGAGGATGCCACTAGAGCA | Start: 818 |
| Tm | 60.037 | 60.032 | End: 1083 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | TGCTCTAGTGGCATCCTCCT | CTAGTGGAGGTGGAGGTGGA | Start: 1064 |
| Tm | 60.032 | 59.959 | End: 1281 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | TCGAGTTCCTGAAACCGTCG | AATACAACCCACCTCCAGCG | Start: 4608 |
| Tm | 60.04 | 60.035 | End: 4851 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | TTTGATGCGCAACCAGAAGC | CGACGGTTTCAGGAACTCGA | Start: 4497 |
| Tm | 60.039 | 60.04 | End: 4627 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | TCCACCTCCACCTCCACTAG | CCGCAGACTACGTGTTGAGT | Start: 1262 |
| Tm | 59.959 | 60.039 | End: 1445 |
| Size | 20 | 20 | 184 |
(ATA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACTTGGGGATCGTCGTTT | AGGAGGATGCCACTAGAGCA | Start: 818 |
| Tm | 60.037 | 60.032 | End: 1083 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | TGCTCTAGTGGCATCCTCCT | CTAGTGGAGGTGGAGGTGGA | Start: 1064 |
| Tm | 60.032 | 59.959 | End: 1281 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | TCGAGTTCCTGAAACCGTCG | AATACAACCCACCTCCAGCG | Start: 4608 |
| Tm | 60.04 | 60.035 | End: 4851 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | TTTGATGCGCAACCAGAAGC | CGACGGTTTCAGGAACTCGA | Start: 4497 |
| Tm | 60.039 | 60.04 | End: 4627 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | TCCACCTCCACCTCCACTAG | CCGCAGACTACGTGTTGAGT | Start: 1262 |
| Tm | 59.959 | 60.039 | End: 1445 |
| Size | 20 | 20 | 184 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACTTGGGGATCGTCGTTT | AGGAGGATGCCACTAGAGCA | Start: 818 |
| Tm | 60.037 | 60.032 | End: 1083 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | TGCTCTAGTGGCATCCTCCT | CTAGTGGAGGTGGAGGTGGA | Start: 1064 |
| Tm | 60.032 | 59.959 | End: 1281 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | TCGAGTTCCTGAAACCGTCG | AATACAACCCACCTCCAGCG | Start: 4608 |
| Tm | 60.04 | 60.035 | End: 4851 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | TTTGATGCGCAACCAGAAGC | CGACGGTTTCAGGAACTCGA | Start: 4497 |
| Tm | 60.039 | 60.04 | End: 4627 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | TCCACCTCCACCTCCACTAG | CCGCAGACTACGTGTTGAGT | Start: 1262 |
| Tm | 59.959 | 60.039 | End: 1445 |
| Size | 20 | 20 | 184 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACTTGGGGATCGTCGTTT | AGGAGGATGCCACTAGAGCA | Start: 818 |
| Tm | 60.037 | 60.032 | End: 1083 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | TGCTCTAGTGGCATCCTCCT | CTAGTGGAGGTGGAGGTGGA | Start: 1064 |
| Tm | 60.032 | 59.959 | End: 1281 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | TCGAGTTCCTGAAACCGTCG | AATACAACCCACCTCCAGCG | Start: 4608 |
| Tm | 60.04 | 60.035 | End: 4851 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | TTTGATGCGCAACCAGAAGC | CGACGGTTTCAGGAACTCGA | Start: 4497 |
| Tm | 60.039 | 60.04 | End: 4627 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | TCCACCTCCACCTCCACTAG | CCGCAGACTACGTGTTGAGT | Start: 1262 |
| Tm | 59.959 | 60.039 | End: 1445 |
| Size | 20 | 20 | 184 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACTTGGGGATCGTCGTTT | AGGAGGATGCCACTAGAGCA | Start: 818 |
| Tm | 60.037 | 60.032 | End: 1083 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | TGCTCTAGTGGCATCCTCCT | CTAGTGGAGGTGGAGGTGGA | Start: 1064 |
| Tm | 60.032 | 59.959 | End: 1281 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | TCGAGTTCCTGAAACCGTCG | AATACAACCCACCTCCAGCG | Start: 4608 |
| Tm | 60.04 | 60.035 | End: 4851 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | TTTGATGCGCAACCAGAAGC | CGACGGTTTCAGGAACTCGA | Start: 4497 |
| Tm | 60.039 | 60.04 | End: 4627 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | TCCACCTCCACCTCCACTAG | CCGCAGACTACGTGTTGAGT | Start: 1262 |
| Tm | 59.959 | 60.039 | End: 1445 |
| Size | 20 | 20 | 184 |
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