ssr_primer
Primer Details for TRINITY_DN12519_c5_g1_i1 |
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| (A)10gggattgcaaaacctttccaaatgctttgtgaaaacccgtttctaagtgaataatttcatataaaaaaaaatgttgcagtctctaaaaaaaattt(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATGTCTTCGAAAGCCTCC | AGAAACGGGTTTTCACAAAGCA | Start: 73 |
| Tm | 59.27 | 59.506 | End: 200 |
| Size | 20 | 22 | 128 |
| Primer 2 details |
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| Sequence | GCATGTCTTCGAAAGCCTCC | TGGAAAGGTTTTGCAATCCCT | Start: 73 |
| Tm | 59.27 | 58.312 | End: 176 |
| Size | 20 | 21 | 104 |
| Primer 3 details |
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| Sequence | GCATGTCTTCGAAAGCCTCC | GAAACGGGTTTTCACAAAGCA | Start: 73 |
| Tm | 59.27 | 58.118 | End: 199 |
| Size | 20 | 21 | 127 |
| Primer 4 details |
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| Sequence | GTGCATGTCTTCGAAAGCCTC | AGAAACGGGTTTTCACAAAGCA | Start: 71 |
| Tm | 59.871 | 59.506 | End: 200 |
| Size | 21 | 22 | 130 |
| Primer 5 details |
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| Sequence | CAGTGCATGTCTTCGAAAGCC | AGAAACGGGTTTTCACAAAGCA | Start: 69 |
| Tm | 60.136 | 59.506 | End: 200 |
| Size | 21 | 22 | 132 |
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