Primer Details for TRINITY_DN12518_c3_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTCTTTTTGCATCACTTTCTCTCT | ACACCCTCATCCCACAATCTG | Start: 19 |
| Tm | 59.68 | 59.717 | End: 167 |
| Size | 26 | 21 | 149 |
| Primer 2 details |
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| Sequence | TGTTCTTTTTGCATCACTTTCTCT | ACACCCTCATCCCACAATCTG | Start: 19 |
| Tm | 57.545 | 59.717 | End: 167 |
| Size | 24 | 21 | 149 |
| Primer 3 details |
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| Sequence | TGTTCTTTTTGCATCACTTTCTCTC | ACACCCTCATCCCACAATCTG | Start: 19 |
| Tm | 58.502 | 59.717 | End: 167 |
| Size | 25 | 21 | 149 |
| Primer 4 details |
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| Sequence | TGTTCTTTTTGCATCACTTTCTCTCT | CACCCTCATCCCACAATCTGA | Start: 19 |
| Tm | 59.68 | 59.439 | End: 166 |
| Size | 26 | 21 | 148 |
| Primer 5 details |
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| Sequence | TGTTCTTTTTGCATCACTTTCTCTCT | ACACCCTCATCCCACAATCT | Start: 19 |
| Tm | 59.68 | 58.326 | End: 167 |
| Size | 26 | 20 | 149 |
