ssr_primer
Primer Details for TRINITY_DN12518_c2_g4_i1 |
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| (CAG)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCCGGTGCACTTCAAGTA | CCACCCATGGTTCACTTCGA | Start: 1053 |
| Tm | 59.966 | 59.964 | End: 1216 |
| Size | 20 | 20 | 164 |
| Primer 2 details |
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| Sequence | TGGAGCCGCTTTTCTGCATA | ATTGGTGTGGTCTCGCATGT | Start: 3064 |
| Tm | 60.036 | 59.963 | End: 3269 |
| Size | 20 | 20 | 206 |
| Primer 3 details |
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| Sequence | CCGCAGTGCTGAGACAACTA | TACTTGAAGTGCACCGGGAC | Start: 938 |
| Tm | 60.038 | 59.966 | End: 1072 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
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| Sequence | TGGAGCCGCTTTTCTGCATA | TCAGATTGGTGTGGTCTCGC | Start: 3064 |
| Tm | 60.036 | 60.037 | End: 3273 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
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| Sequence | ACATGCGAGACCACACCAAT | TTAATGTGCGAGCCTCTGCA | Start: 3250 |
| Tm | 59.963 | 60.036 | End: 3382 |
| Size | 20 | 20 | 133 |
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