ssr_primer
Primer Details for TRINITY_DN12517_c1_g4_i1 |
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| (AT)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTGAGAAGCCACACTCGAA | ACTAGTGTGAAGCCCGAAGC | Start: 397 |
| Tm | 60.32 | 60.037 | End: 528 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
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| Sequence | ATCATGTTCTCAGGCCTGCC | TGGATGCCGAAGCCTTTTCA | Start: 1509 |
| Tm | 60.107 | 60.251 | End: 1638 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
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| Sequence | TGCGAGTGGTCCCTTTTGTT | TGGATGCCGAAGCCTTTTCA | Start: 1476 |
| Tm | 60.107 | 60.251 | End: 1638 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
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| Sequence | TTGCGAGTGGTCCCTTTTGT | TGGATGCCGAAGCCTTTTCA | Start: 1475 |
| Tm | 60.107 | 60.251 | End: 1638 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
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| Sequence | ATCATGTTCTCAGGCCTGCC | CGTGTCACAGCTCCCATGAA | Start: 1509 |
| Tm | 60.107 | 60.321 | End: 1670 |
| Size | 20 | 20 | 162 |
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