ssr_primer
Primer Details for TRINITY_DN12500_c1_g9_i1 |
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| (T)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCCCAAGCCCATACACAT | TGATGAGTCCAGGGTCGGAT | Start: 391 |
| Tm | 60.033 | 60.032 | End: 518 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
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| Sequence | GTCATCGTGGGTCGGTCTTT | ATGTGTATGGGCTTGGGGTG | Start: 155 |
| Tm | 60.038 | 60.033 | End: 410 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
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| Sequence | GTCTTCTCCGGGTAAACGCA | AGCTCTCGGTTCAGCCTCTA | Start: 2142 |
| Tm | 60.038 | 60.034 | End: 2389 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
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| Sequence | GTAGCCGCCGGACCTTATAC | AGCTCTCGGTTCAGCCTCTA | Start: 2197 |
| Tm | 60.039 | 60.034 | End: 2389 |
| Size | 20 | 20 | 193 |
| Primer 5 details |
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| Sequence | GTATAGTGTAGCCGCCGGAC | AGCTCTCGGTTCAGCCTCTA | Start: 2190 |
| Tm | 60.04 | 60.034 | End: 2389 |
| Size | 20 | 20 | 200 |
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