ssr_primer
Primer Details for TRINITY_DN12499_c0_g4_i3 |
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| (TTTC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCCGTTCCCCTTTTGTTG | GTAGGGTTGGGGGAAAGTGG | Start: 182 |
| Tm | 59.968 | 59.961 | End: 394 |
| Size | 20 | 20 | 213 |
| Primer 2 details |
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| Sequence | AAGGCTCTCCTCTTGTTGCC | TTCTACCGCCATTGCCACAT | Start: 783 |
| Tm | 59.963 | 60.035 | End: 1060 |
| Size | 20 | 20 | 278 |
| Primer 3 details |
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| Sequence | CACGTATGACCCGACGGAAT | TTCTACCGCCATTGCCACAT | Start: 829 |
| Tm | 59.898 | 60.035 | End: 1060 |
| Size | 20 | 20 | 232 |
| Primer 4 details |
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| Sequence | CTGCCGTTCCCCTTTTGTTG | AATCGTGGAACTCGTCGTCC | Start: 182 |
| Tm | 59.968 | 60.109 | End: 350 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
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| Sequence | AGAGGACGAAAGCTGTGTGG | CATGAAAGCAGCAGCCACAG | Start: 1101 |
| Tm | 59.966 | 60.109 | End: 1204 |
| Size | 20 | 20 | 104 |
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