ssr_primer
Primer Details for TRINITY_DN12490_c3_g5_i3 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCGCCACTCTCCTCTTCCT | GTTAATGTGGCCACCGGAGA | Start: 242 |
| Tm | 60.033 | 60.036 | End: 421 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
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| Sequence | CATCGCCACTCTCCTCTTCC | GTTAATGTGGCCACCGGAGA | Start: 241 |
| Tm | 59.896 | 60.036 | End: 421 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
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| Sequence | CCAATTGGGTTTGCCGTGTT | TTTAATGGCGCCAGCTCTGA | Start: 815 |
| Tm | 59.894 | 60.036 | End: 1024 |
| Size | 20 | 20 | 210 |
| Primer 4 details |
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| Sequence | AAGTTTCGGAGCTGCTGTCA | TTTAATGGCGCCAGCTCTGA | Start: 889 |
| Tm | 59.893 | 60.036 | End: 1024 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
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| Sequence | TCAGAGCTGGCGCCATTAAA | GGCAAGATGAGAGCAGAGCA | Start: 1005 |
| Tm | 60.036 | 60.108 | End: 1209 |
| Size | 20 | 20 | 205 |
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