ssr_primer
Primer Details for TRINITY_DN12489_c1_g1_i2 |
|---|
| (AAT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAGCCAGTTGGGGGAAGTG | CGTCGACTGATGGCTGTCAT | Start: 534 |
| Tm | 60.035 | 60.179 | End: 642 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
|---|
| Sequence | TCATCTAGCCAGTTGGGGGA | CGTCGACTGATGGCTGTCAT | Start: 530 |
| Tm | 59.956 | 60.179 | End: 642 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | GGGACACGAATGAACCGGTA | AACCACCAAAAGGGAGGGTG | Start: 1481 |
| Tm | 59.753 | 60.106 | End: 1613 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | TCTAGCCAGTTGGGGGAAGT | CGTCGACTGATGGCTGTCAT | Start: 533 |
| Tm | 60.179 | 60.179 | End: 642 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | CGTCATCTAGCCAGTTGGGG | CGTCGACTGATGGCTGTCAT | Start: 528 |
| Tm | 60.179 | 60.179 | End: 642 |
| Size | 20 | 20 | 115 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAGCCAGTTGGGGGAAGTG | CGTCGACTGATGGCTGTCAT | Start: 534 |
| Tm | 60.035 | 60.179 | End: 642 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
|---|
| Sequence | TCATCTAGCCAGTTGGGGGA | CGTCGACTGATGGCTGTCAT | Start: 530 |
| Tm | 59.956 | 60.179 | End: 642 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | GGGACACGAATGAACCGGTA | AACCACCAAAAGGGAGGGTG | Start: 1481 |
| Tm | 59.753 | 60.106 | End: 1613 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | TCTAGCCAGTTGGGGGAAGT | CGTCGACTGATGGCTGTCAT | Start: 533 |
| Tm | 60.179 | 60.179 | End: 642 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | CGTCATCTAGCCAGTTGGGG | CGTCGACTGATGGCTGTCAT | Start: 528 |
| Tm | 60.179 | 60.179 | End: 642 |
| Size | 20 | 20 | 115 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAGCCAGTTGGGGGAAGTG | CGTCGACTGATGGCTGTCAT | Start: 534 |
| Tm | 60.035 | 60.179 | End: 642 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
|---|
| Sequence | TCATCTAGCCAGTTGGGGGA | CGTCGACTGATGGCTGTCAT | Start: 530 |
| Tm | 59.956 | 60.179 | End: 642 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | GGGACACGAATGAACCGGTA | AACCACCAAAAGGGAGGGTG | Start: 1481 |
| Tm | 59.753 | 60.106 | End: 1613 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | TCTAGCCAGTTGGGGGAAGT | CGTCGACTGATGGCTGTCAT | Start: 533 |
| Tm | 60.179 | 60.179 | End: 642 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | CGTCATCTAGCCAGTTGGGG | CGTCGACTGATGGCTGTCAT | Start: 528 |
| Tm | 60.179 | 60.179 | End: 642 |
| Size | 20 | 20 | 115 |
(AT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAGCCAGTTGGGGGAAGTG | CGTCGACTGATGGCTGTCAT | Start: 534 |
| Tm | 60.035 | 60.179 | End: 642 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
|---|
| Sequence | TCATCTAGCCAGTTGGGGGA | CGTCGACTGATGGCTGTCAT | Start: 530 |
| Tm | 59.956 | 60.179 | End: 642 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | GGGACACGAATGAACCGGTA | AACCACCAAAAGGGAGGGTG | Start: 1481 |
| Tm | 59.753 | 60.106 | End: 1613 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | TCTAGCCAGTTGGGGGAAGT | CGTCGACTGATGGCTGTCAT | Start: 533 |
| Tm | 60.179 | 60.179 | End: 642 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | CGTCATCTAGCCAGTTGGGG | CGTCGACTGATGGCTGTCAT | Start: 528 |
| Tm | 60.179 | 60.179 | End: 642 |
| Size | 20 | 20 | 115 |
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