ssr_primer
Primer Details for TRINITY_DN12486_c1_g1_i1 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGACATGTGTGGTCGACC | CAACCCTACATGTGGCTGGT | Start: 1044 |
| Tm | 59.966 | 59.962 | End: 1214 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | ACCAGCCACATGTAGGGTTG | ACAAGGAACAGCCACAACCA | Start: 1195 |
| Tm | 59.962 | 60.034 | End: 1415 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
|---|
| Sequence | ACAGACATGTGTGGTCGACC | TTTGGAAAGGTGGGCGAGAA | Start: 1044 |
| Tm | 59.966 | 59.816 | End: 1177 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | ACAGACATGTGTGGTCGACC | TTTTGGAAAGGTGGGCGAGA | Start: 1044 |
| Tm | 59.966 | 59.816 | End: 1178 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | TCTCGCCCACCTTTCCAAAA | ACAAGGAACAGCCACAACCA | Start: 1159 |
| Tm | 59.816 | 60.034 | End: 1415 |
| Size | 20 | 20 | 257 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGACATGTGTGGTCGACC | CAACCCTACATGTGGCTGGT | Start: 1044 |
| Tm | 59.966 | 59.962 | End: 1214 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | ACCAGCCACATGTAGGGTTG | ACAAGGAACAGCCACAACCA | Start: 1195 |
| Tm | 59.962 | 60.034 | End: 1415 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
|---|
| Sequence | ACAGACATGTGTGGTCGACC | TTTGGAAAGGTGGGCGAGAA | Start: 1044 |
| Tm | 59.966 | 59.816 | End: 1177 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | ACAGACATGTGTGGTCGACC | TTTTGGAAAGGTGGGCGAGA | Start: 1044 |
| Tm | 59.966 | 59.816 | End: 1178 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | TCTCGCCCACCTTTCCAAAA | ACAAGGAACAGCCACAACCA | Start: 1159 |
| Tm | 59.816 | 60.034 | End: 1415 |
| Size | 20 | 20 | 257 |
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