ssr_primer
Primer Details for TRINITY_DN12479_c0_g1_i11 |
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| (T)10atatgttttgtctatttgtaatcc(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGGCGAGTAGGACGTGATG | ACTTTGACGGATCATGCGGT | Start: 1986 |
| Tm | 59.97 | 60.036 | End: 2257 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
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| Sequence | GAGGCGAGTAGGACGTGATG | TTGGGACGATTGGCATCACA | Start: 1986 |
| Tm | 59.97 | 59.963 | End: 2105 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
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| Sequence | GGCCTTGATTCCGACCTTGA | GGCCCCTTTCCATTCGAGAA | Start: 2833 |
| Tm | 60.035 | 60.034 | End: 2959 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
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| Sequence | CTGGTCCGAGGCAGAAAAGT | GGCCCCTTTCCATTCGAGAA | Start: 2750 |
| Tm | 59.965 | 60.034 | End: 2959 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
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| Sequence | CAAGGGCTTGACAGGTCGAT | GGCCCCTTTCCATTCGAGAA | Start: 2775 |
| Tm | 60.036 | 60.034 | End: 2959 |
| Size | 20 | 20 | 185 |
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