ssr_primer
Primer Details for TRINITY_DN12478_c3_g5_i8 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATGGAAATGGCGGGGGAAT | AAGAAGATGGTGGCTGGCTC | Start: 188 |
| Tm | 60.031 | 60.035 | End: 305 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
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| Sequence | AATGGAAATGGCGGGGGAAT | GAAGAAGATGGTGGCTGGCT | Start: 188 |
| Tm | 60.031 | 60.035 | End: 306 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
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| Sequence | ATCTTTCATCGCCGATCGCT | AAAGGAAGTAGAGTGCGGGC | Start: 434 |
| Tm | 59.967 | 60.036 | End: 546 |
| Size | 20 | 20 | 113 |
| Primer 4 details |
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| Sequence | ATCTTTCATCGCCGATCGCT | GCATTTCCGCCACCAACAAT | Start: 434 |
| Tm | 59.967 | 60.037 | End: 668 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
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| Sequence | AGCCAGCCACCATCTTCTTC | AAAGGAAGTAGAGTGCGGGC | Start: 287 |
| Tm | 60.035 | 60.036 | End: 546 |
| Size | 20 | 20 | 260 |
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