ssr_primer
Primer Details for TRINITY_DN12468_c2_g1_i1 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTAACAGGATGCCCAACT | AAGCAGATCCTTTGTCCCCG | Start: 925 |
| Tm | 60.034 | 60.035 | End: 1038 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | CTTGGAGACTGCACGAGTGT | CCCGACAATTGACCCCTCAA | Start: 1677 |
| Tm | 59.967 | 59.962 | End: 1830 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
|---|
| Sequence | CTTGGAGACTGCACGAGTGT | AACCCGACAATTGACCCCTC | Start: 1677 |
| Tm | 59.967 | 59.962 | End: 1832 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | CTCCGCTAAAAGTGGCCAGA | AGCCAAATGATCAAGCCGGA | Start: 434 |
| Tm | 60.036 | 60.034 | End: 605 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
|---|
| Sequence | TCTCCGCTAAAAGTGGCCAG | AGCCAAATGATCAAGCCGGA | Start: 433 |
| Tm | 60.036 | 60.034 | End: 605 |
| Size | 20 | 20 | 173 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTAACAGGATGCCCAACT | AAGCAGATCCTTTGTCCCCG | Start: 925 |
| Tm | 60.034 | 60.035 | End: 1038 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | CTTGGAGACTGCACGAGTGT | CCCGACAATTGACCCCTCAA | Start: 1677 |
| Tm | 59.967 | 59.962 | End: 1830 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
|---|
| Sequence | CTTGGAGACTGCACGAGTGT | AACCCGACAATTGACCCCTC | Start: 1677 |
| Tm | 59.967 | 59.962 | End: 1832 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | CTCCGCTAAAAGTGGCCAGA | AGCCAAATGATCAAGCCGGA | Start: 434 |
| Tm | 60.036 | 60.034 | End: 605 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
|---|
| Sequence | TCTCCGCTAAAAGTGGCCAG | AGCCAAATGATCAAGCCGGA | Start: 433 |
| Tm | 60.036 | 60.034 | End: 605 |
| Size | 20 | 20 | 173 |
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