ssr_primer
Primer Details for TRINITY_DN12467_c3_g1_i3 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATCGACATGACAATGGGC | TCACGGCGCGATCATATGAA | Start: 808 |
| Tm | 59.97 | 59.969 | End: 1017 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
|---|
| Sequence | CTTGCCTGAGCCTCAGTTCA | TCACGGCGCGATCATATGAA | Start: 908 |
| Tm | 59.964 | 59.969 | End: 1017 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | AATCCACAAGCGAGTCCTGG | ATGCATACCATCTCTGCCGG | Start: 3005 |
| Tm | 60.036 | 59.964 | End: 3114 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
|---|
| Sequence | AAGGCTCGTGGTGTAAGTGG | CCTCCCAGAAGTGCACACAT | Start: 3964 |
| Tm | 59.965 | 59.963 | End: 4159 |
| Size | 20 | 20 | 196 |
| Primer 5 details |
|---|
| Sequence | TTCATATGATCGCGCCGTGA | TAATCATGCGGCGGCTTTTG | Start: 998 |
| Tm | 59.969 | 59.9 | End: 1102 |
| Size | 20 | 20 | 105 |
(TG)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATCGACATGACAATGGGC | TCACGGCGCGATCATATGAA | Start: 808 |
| Tm | 59.97 | 59.969 | End: 1017 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
|---|
| Sequence | CTTGCCTGAGCCTCAGTTCA | TCACGGCGCGATCATATGAA | Start: 908 |
| Tm | 59.964 | 59.969 | End: 1017 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | AATCCACAAGCGAGTCCTGG | ATGCATACCATCTCTGCCGG | Start: 3005 |
| Tm | 60.036 | 59.964 | End: 3114 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
|---|
| Sequence | AAGGCTCGTGGTGTAAGTGG | CCTCCCAGAAGTGCACACAT | Start: 3964 |
| Tm | 59.965 | 59.963 | End: 4159 |
| Size | 20 | 20 | 196 |
| Primer 5 details |
|---|
| Sequence | TTCATATGATCGCGCCGTGA | TAATCATGCGGCGGCTTTTG | Start: 998 |
| Tm | 59.969 | 59.9 | End: 1102 |
| Size | 20 | 20 | 105 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATCGACATGACAATGGGC | TCACGGCGCGATCATATGAA | Start: 808 |
| Tm | 59.97 | 59.969 | End: 1017 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
|---|
| Sequence | CTTGCCTGAGCCTCAGTTCA | TCACGGCGCGATCATATGAA | Start: 908 |
| Tm | 59.964 | 59.969 | End: 1017 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | AATCCACAAGCGAGTCCTGG | ATGCATACCATCTCTGCCGG | Start: 3005 |
| Tm | 60.036 | 59.964 | End: 3114 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
|---|
| Sequence | AAGGCTCGTGGTGTAAGTGG | CCTCCCAGAAGTGCACACAT | Start: 3964 |
| Tm | 59.965 | 59.963 | End: 4159 |
| Size | 20 | 20 | 196 |
| Primer 5 details |
|---|
| Sequence | TTCATATGATCGCGCCGTGA | TAATCATGCGGCGGCTTTTG | Start: 998 |
| Tm | 59.969 | 59.9 | End: 1102 |
| Size | 20 | 20 | 105 |
(ATT)6tatttatttgatgacaaagtagtatttttttttattctctttatttttctga(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATCGACATGACAATGGGC | TCACGGCGCGATCATATGAA | Start: 808 |
| Tm | 59.97 | 59.969 | End: 1017 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
|---|
| Sequence | CTTGCCTGAGCCTCAGTTCA | TCACGGCGCGATCATATGAA | Start: 908 |
| Tm | 59.964 | 59.969 | End: 1017 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | AATCCACAAGCGAGTCCTGG | ATGCATACCATCTCTGCCGG | Start: 3005 |
| Tm | 60.036 | 59.964 | End: 3114 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
|---|
| Sequence | AAGGCTCGTGGTGTAAGTGG | CCTCCCAGAAGTGCACACAT | Start: 3964 |
| Tm | 59.965 | 59.963 | End: 4159 |
| Size | 20 | 20 | 196 |
| Primer 5 details |
|---|
| Sequence | TTCATATGATCGCGCCGTGA | TAATCATGCGGCGGCTTTTG | Start: 998 |
| Tm | 59.969 | 59.9 | End: 1102 |
| Size | 20 | 20 | 105 |
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