ssr_primer
Primer Details for TRINITY_DN12462_c0_g4_i3 |
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| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCACCACTCCTGAGGTAAC | AACGCCCTGGATTCTGGAAG | Start: 1343 |
| Tm | 60.036 | 60.035 | End: 1574 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
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| Sequence | CTCTTGGCACCACTCCTGAG | AACGCCCTGGATTCTGGAAG | Start: 1338 |
| Tm | 60.037 | 60.035 | End: 1574 |
| Size | 20 | 20 | 237 |
| Primer 3 details |
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| Sequence | CTTCCAGAATCCAGGGCGTT | TTCTGGGGTCTGCCTCCATA | Start: 1555 |
| Tm | 60.035 | 59.956 | End: 1817 |
| Size | 20 | 20 | 263 |
| Primer 4 details |
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| Sequence | TATGGAGGCAGACCCCAGAA | CGCTTGAACCTGGCTAGTGA | Start: 1798 |
| Tm | 59.956 | 60.037 | End: 2026 |
| Size | 20 | 20 | 229 |
| Primer 5 details |
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| Sequence | CGGAGGAAGCTGAGGAGTTG | TGGAATGGTGGACGTTGGAG | Start: 741 |
| Tm | 60.108 | 59.964 | End: 886 |
| Size | 20 | 20 | 146 |
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