ssr_primer
Primer Details for TRINITY_DN12460_c2_g4_i5 |
|---|
| (TTC)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAGCAACACGTGATAGCG | AAGGGAAGAAGAGGCATGGC | Start: 1470 |
| Tm | 59.976 | 60.034 | End: 1635 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
|---|
| Sequence | GCCTCTTTGGGTTCGAGACA | CGCTATCACGTGTTGCTTCG | Start: 1353 |
| Tm | 59.965 | 59.976 | End: 1489 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
|---|
| Sequence | CGAAGCAACACGTGATAGCG | GGCATGGCCTGAAACACTTG | Start: 1470 |
| Tm | 59.976 | 60.038 | End: 1623 |
| Size | 20 | 20 | 154 |
| Primer 4 details |
|---|
| Sequence | CGAAGCAACACGTGATAGCG | TGTGCTTCTCTCGTCGGAAC | Start: 1470 |
| Tm | 59.976 | 60.039 | End: 1694 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
|---|
| Sequence | GGAGGATGTTTCCATGGCCA | AAGGGAAGAAGAGGCATGGC | Start: 1412 |
| Tm | 60.033 | 60.034 | End: 1635 |
| Size | 20 | 20 | 224 |
(GA)10aggcaaattagtattttaaaaaagtgtaattattattattaattaaaaaaatataggagtagtttttgggaggtaaataaggtaagt(GA)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAGCAACACGTGATAGCG | AAGGGAAGAAGAGGCATGGC | Start: 1470 |
| Tm | 59.976 | 60.034 | End: 1635 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
|---|
| Sequence | GCCTCTTTGGGTTCGAGACA | CGCTATCACGTGTTGCTTCG | Start: 1353 |
| Tm | 59.965 | 59.976 | End: 1489 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
|---|
| Sequence | CGAAGCAACACGTGATAGCG | GGCATGGCCTGAAACACTTG | Start: 1470 |
| Tm | 59.976 | 60.038 | End: 1623 |
| Size | 20 | 20 | 154 |
| Primer 4 details |
|---|
| Sequence | CGAAGCAACACGTGATAGCG | TGTGCTTCTCTCGTCGGAAC | Start: 1470 |
| Tm | 59.976 | 60.039 | End: 1694 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
|---|
| Sequence | GGAGGATGTTTCCATGGCCA | AAGGGAAGAAGAGGCATGGC | Start: 1412 |
| Tm | 60.033 | 60.034 | End: 1635 |
| Size | 20 | 20 | 224 |
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