ssr_primer
Primer Details for TRINITY_DN12434_c1_g3_i2 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGGCTCGGGCAACTTTTC | AATCAGCAACACCACCACCA | Start: 2185 |
| Tm | 60.039 | 60.106 | End: 2333 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | TGGTGGTGGTGTTGCTGATT | GCCCAACAACAACCCAACTC | Start: 2314 |
| Tm | 60.106 | 59.896 | End: 2497 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
|---|
| Sequence | CAAGGCTCGGGCAACTTTTC | CCACCACCACCAACAACAAC | Start: 2185 |
| Tm | 60.039 | 59.825 | End: 2322 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | AACCAGAGTGAGGGCTGAGA | GACAGTGGAGGAGTTGGGTG | Start: 1256 |
| Tm | 60.179 | 59.965 | End: 1386 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | CACCCAACTCCTCCACTGTC | TTTCCCCTTCGGTGGGTTTC | Start: 1367 |
| Tm | 59.965 | 60.179 | End: 1512 |
| Size | 20 | 20 | 146 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGGCTCGGGCAACTTTTC | AATCAGCAACACCACCACCA | Start: 2185 |
| Tm | 60.039 | 60.106 | End: 2333 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | TGGTGGTGGTGTTGCTGATT | GCCCAACAACAACCCAACTC | Start: 2314 |
| Tm | 60.106 | 59.896 | End: 2497 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
|---|
| Sequence | CAAGGCTCGGGCAACTTTTC | CCACCACCACCAACAACAAC | Start: 2185 |
| Tm | 60.039 | 59.825 | End: 2322 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | AACCAGAGTGAGGGCTGAGA | GACAGTGGAGGAGTTGGGTG | Start: 1256 |
| Tm | 60.179 | 59.965 | End: 1386 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | CACCCAACTCCTCCACTGTC | TTTCCCCTTCGGTGGGTTTC | Start: 1367 |
| Tm | 59.965 | 60.179 | End: 1512 |
| Size | 20 | 20 | 146 |
(AGA)7aa(AAG)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGGCTCGGGCAACTTTTC | AATCAGCAACACCACCACCA | Start: 2185 |
| Tm | 60.039 | 60.106 | End: 2333 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | TGGTGGTGGTGTTGCTGATT | GCCCAACAACAACCCAACTC | Start: 2314 |
| Tm | 60.106 | 59.896 | End: 2497 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
|---|
| Sequence | CAAGGCTCGGGCAACTTTTC | CCACCACCACCAACAACAAC | Start: 2185 |
| Tm | 60.039 | 59.825 | End: 2322 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | AACCAGAGTGAGGGCTGAGA | GACAGTGGAGGAGTTGGGTG | Start: 1256 |
| Tm | 60.179 | 59.965 | End: 1386 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | CACCCAACTCCTCCACTGTC | TTTCCCCTTCGGTGGGTTTC | Start: 1367 |
| Tm | 59.965 | 60.179 | End: 1512 |
| Size | 20 | 20 | 146 |
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