Primer Details for TRINITY_DN12434_c1_g10_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCTTGGATTGTTTTCTATACAACA | AGCTAAGGTAGGGGGAGACA | Start: 16 |
| Tm | 57.301 | 58.984 | End: 161 |
| Size | 26 | 20 | 146 |
| Primer 2 details |
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| Sequence | TGTCTCCCCCTACCTTAGCT | AGGATATAGAAAAACAAAAAGGGGTT | Start: 142 |
| Tm | 58.984 | 57.264 | End: 275 |
| Size | 20 | 26 | 134 |
| Primer 3 details |
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| Sequence | TGTCTCCCCCTACCTTAGCT | AAGGATATAGAAAAACAAAAAGGGGT | Start: 142 |
| Tm | 58.984 | 57.264 | End: 276 |
| Size | 20 | 26 | 135 |
| Primer 4 details |
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| Sequence | TCCTTGGATTGTTTTCTATACAACAT | AGCTAAGGTAGGGGGAGACA | Start: 17 |
| Tm | 57.122 | 58.984 | End: 161 |
| Size | 26 | 20 | 145 |
| Primer 5 details |
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| Sequence | TTCCTTGGATTGTTTTCTATACAACA | AAGCTAAGGTAGGGGGAGACA | Start: 16 |
| Tm | 57.301 | 59.635 | End: 162 |
| Size | 26 | 21 | 147 |
