ssr_primer
Primer Details for TRINITY_DN12423_c0_g3_i7 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCAGCCTTCGCATCAAAAA | TGTTGACCTTACCGTCAGGC | Start: 600 |
| Tm | 60.038 | 59.966 | End: 875 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
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| Sequence | TAACCTTCTTGGCACCACCC | GACAAGGACAAAGCTGCTGC | Start: 1656 |
| Tm | 59.888 | 60.04 | End: 1887 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
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| Sequence | CCAAAGCCTTCCCTTCCCTT | TTTTTGATGCGAAGGCTGGC | Start: 432 |
| Tm | 59.885 | 60.038 | End: 619 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
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| Sequence | CCAAAGCCTTCCCTTCCCTT | GCTGGCATTGCTTTGAACGA | Start: 432 |
| Tm | 59.885 | 60.039 | End: 605 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
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| Sequence | TAACCTTCTTGGCACCACCC | GGAGGTTGGAGCCGACTATG | Start: 1656 |
| Tm | 59.888 | 59.895 | End: 1933 |
| Size | 20 | 20 | 278 |
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