ssr_primer
Primer Details for TRINITY_DN12421_c1_g1_i7 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTCGTCATGGGTCTCGTG | CCCCGACCAAAAACAGCAAG | Start: 1090 |
| Tm | 60.039 | 59.968 | End: 1365 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
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| Sequence | CAGCAAGTACCGAACTGGGT | CACGAGACCCATGACGAACA | Start: 981 |
| Tm | 59.965 | 60.039 | End: 1109 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
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| Sequence | ACCCTCAACTCTATGGGGCT | AGGCCTGAAATGTCGACGTT | Start: 4807 |
| Tm | 59.955 | 59.965 | End: 4957 |
| Size | 20 | 20 | 151 |
| Primer 4 details |
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| Sequence | ACCCTCAACTCTATGGGGCT | AAGGCCTGAAATGTCGACGT | Start: 4807 |
| Tm | 59.955 | 59.965 | End: 4958 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
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| Sequence | ACCCTCAACTCTATGGGGCT | GCCCAAAATGCAAAGAGGGG | Start: 4807 |
| Tm | 59.955 | 60.036 | End: 4933 |
| Size | 20 | 20 | 127 |
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