Primer Details for TRINITY_DN12413_c6_g1_i1 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAACAAGAATGAAACTAGAAGACACA | TCTTCATGATTATGGAGAGTGTTCC | Start: 83 |
| Tm | 59.447 | 58.414 | End: 215 |
| Size | 27 | 25 | 133 |
| Primer 2 details |
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| Sequence | ACAAGAATGAAACTAGAAGACACATGT | TCTTCATGATTATGGAGAGTGTTCC | Start: 86 |
| Tm | 59.283 | 58.414 | End: 215 |
| Size | 27 | 25 | 130 |
| Primer 3 details |
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| Sequence | ACAACAAGAATGAAACTAGAAGACACA | CTTCATGATTATGGAGAGTGTTCC | Start: 83 |
| Tm | 59.447 | 57.142 | End: 214 |
| Size | 27 | 24 | 132 |
| Primer 4 details |
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| Sequence | ACACATGTCTTCTATTATGGTTGAGA | TCTTCATGATTATGGAGAGTGTTCC | Start: 105 |
| Tm | 58.149 | 58.414 | End: 215 |
| Size | 26 | 25 | 111 |
| Primer 5 details |
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| Sequence | AGAATGAAACTAGAAGACACATGTC | TCTTCATGATTATGGAGAGTGTTCC | Start: 89 |
| Tm | 57.102 | 58.414 | End: 215 |
| Size | 25 | 25 | 127 |
