ssr_primer
Primer Details for TRINITY_DN12413_c1_g2_i1 |
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| (T)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCATTGAACCCGGAAGACG | TCCCTGAAAATAGCGAATCGAGA | Start: 75 |
| Tm | 60.037 | 59.616 | End: 214 |
| Size | 20 | 23 | 140 |
| Primer 2 details |
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| Sequence | AAGACGGGGAGATCCACAGA | TCCCTGAAAATAGCGAATCGAGA | Start: 89 |
| Tm | 59.959 | 59.616 | End: 214 |
| Size | 20 | 23 | 126 |
| Primer 3 details |
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| Sequence | TCCATTGAACCCGGAAGACG | TGAAGCTGAAGAAATGGGGAA | Start: 75 |
| Tm | 60.037 | 57.476 | End: 175 |
| Size | 20 | 21 | 101 |
| Primer 4 details |
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| Sequence | TCCATTGAACCCGGAAGACG | TTGAAGCTGAAGAAATGGGGA | Start: 75 |
| Tm | 60.037 | 57.476 | End: 176 |
| Size | 20 | 21 | 102 |
| Primer 5 details |
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| Sequence | AGACGGGGAGATCCACAGAT | TCCCTGAAAATAGCGAATCGAGA | Start: 90 |
| Tm | 59.737 | 59.616 | End: 214 |
| Size | 20 | 23 | 125 |
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