ssr_primer
Primer Details for TRINITY_DN12408_c2_g7_i2 |
|---|
| (ATTAGG)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGAGTGAGAAGAAAGCCT | TCGATGTCGGTTTGGGGAAG | Start: 161 |
| Tm | 60.037 | 60.037 | End: 381 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | ATCCTTTCGCCGGTTTTCCT | CGGTTGAGTGGAGGCATCTT | Start: 1004 |
| Tm | 59.962 | 60.036 | End: 1111 |
| Size | 20 | 20 | 108 |
| Primer 3 details |
|---|
| Sequence | ACGTGCAAACCGATCTCGAT | TCGATGTCGGTTTGGGGAAG | Start: 276 |
| Tm | 60.109 | 60.037 | End: 381 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | GGCGAGTGAGAAGAAAGCCT | ATCGAGATCGGTTTGCACGT | Start: 161 |
| Tm | 60.037 | 60.109 | End: 295 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | TTTTCAACCAAACCGCCACC | TCGATGTCGGTTTGGGGAAG | Start: 231 |
| Tm | 59.824 | 60.037 | End: 381 |
| Size | 20 | 20 | 151 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGAGTGAGAAGAAAGCCT | TCGATGTCGGTTTGGGGAAG | Start: 161 |
| Tm | 60.037 | 60.037 | End: 381 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | ATCCTTTCGCCGGTTTTCCT | CGGTTGAGTGGAGGCATCTT | Start: 1004 |
| Tm | 59.962 | 60.036 | End: 1111 |
| Size | 20 | 20 | 108 |
| Primer 3 details |
|---|
| Sequence | ACGTGCAAACCGATCTCGAT | TCGATGTCGGTTTGGGGAAG | Start: 276 |
| Tm | 60.109 | 60.037 | End: 381 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | GGCGAGTGAGAAGAAAGCCT | ATCGAGATCGGTTTGCACGT | Start: 161 |
| Tm | 60.037 | 60.109 | End: 295 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | TTTTCAACCAAACCGCCACC | TCGATGTCGGTTTGGGGAAG | Start: 231 |
| Tm | 59.824 | 60.037 | End: 381 |
| Size | 20 | 20 | 151 |
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