ssr_primer
Primer Details for TRINITY_DN12389_c2_g2_i7 |
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| (TCT)10ctctctctgtactcttcactcact(CA)9(CT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCAACTCACGCACACCTC | GTGGCATTGAGGATGGGTCA | Start: 122 |
| Tm | 59.968 | 60.034 | End: 319 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
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| Sequence | CTCTCAACTCACGCACACCT | GTGGCATTGAGGATGGGTCA | Start: 121 |
| Tm | 59.967 | 60.034 | End: 319 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | TGCGGAAATGAGCAACTCCT | GTTCAGAACGGGAGGGAAGG | Start: 1335 |
| Tm | 59.963 | 60.036 | End: 1561 |
| Size | 20 | 20 | 227 |
| Primer 4 details |
|---|
| Sequence | TATCACGTGGGCTTTGGCTT | CAGGAGTGCCCCACTGTAAG | Start: 2594 |
| Tm | 59.962 | 60.036 | End: 2802 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | TTATCACGTGGGCTTTGGCT | CAGGAGTGCCCCACTGTAAG | Start: 2593 |
| Tm | 59.962 | 60.036 | End: 2802 |
| Size | 20 | 20 | 210 |
(TC)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCAACTCACGCACACCTC | GTGGCATTGAGGATGGGTCA | Start: 122 |
| Tm | 59.968 | 60.034 | End: 319 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | CTCTCAACTCACGCACACCT | GTGGCATTGAGGATGGGTCA | Start: 121 |
| Tm | 59.967 | 60.034 | End: 319 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | TGCGGAAATGAGCAACTCCT | GTTCAGAACGGGAGGGAAGG | Start: 1335 |
| Tm | 59.963 | 60.036 | End: 1561 |
| Size | 20 | 20 | 227 |
| Primer 4 details |
|---|
| Sequence | TATCACGTGGGCTTTGGCTT | CAGGAGTGCCCCACTGTAAG | Start: 2594 |
| Tm | 59.962 | 60.036 | End: 2802 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | TTATCACGTGGGCTTTGGCT | CAGGAGTGCCCCACTGTAAG | Start: 2593 |
| Tm | 59.962 | 60.036 | End: 2802 |
| Size | 20 | 20 | 210 |
(T)25: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCAACTCACGCACACCTC | GTGGCATTGAGGATGGGTCA | Start: 122 |
| Tm | 59.968 | 60.034 | End: 319 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | CTCTCAACTCACGCACACCT | GTGGCATTGAGGATGGGTCA | Start: 121 |
| Tm | 59.967 | 60.034 | End: 319 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | TGCGGAAATGAGCAACTCCT | GTTCAGAACGGGAGGGAAGG | Start: 1335 |
| Tm | 59.963 | 60.036 | End: 1561 |
| Size | 20 | 20 | 227 |
| Primer 4 details |
|---|
| Sequence | TATCACGTGGGCTTTGGCTT | CAGGAGTGCCCCACTGTAAG | Start: 2594 |
| Tm | 59.962 | 60.036 | End: 2802 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | TTATCACGTGGGCTTTGGCT | CAGGAGTGCCCCACTGTAAG | Start: 2593 |
| Tm | 59.962 | 60.036 | End: 2802 |
| Size | 20 | 20 | 210 |
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