ssr_primer
Primer Details for TRINITY_DN12387_c2_g1_i28 |
|---|
| (CT)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGAATCCAAGGCAGCCAGA | CCCACACAACAACATGGCAG | Start: 350 |
| Tm | 60.035 | 59.968 | End: 477 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
|---|
| Sequence | AATGAAGGTTTTGCGTGGCC | TCGTCGCTTGGGTTTGATGA | Start: 2377 |
| Tm | 59.967 | 59.966 | End: 2579 |
| Size | 20 | 20 | 203 |
| Primer 3 details |
|---|
| Sequence | TCATCAAACCCAAGCGACGA | CGGAACAGGGAGAAAGCCAT | Start: 2560 |
| Tm | 59.966 | 60.035 | End: 2720 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | GAGTGTGCATCCGGGTAACA | GGCCACGCAAAACCTTCATT | Start: 2266 |
| Tm | 60.037 | 59.967 | End: 2396 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | CCACCGTGTTTCCGAGAGAA | CGTCTCCCTCGTGAAGCTTT | Start: 3325 |
| Tm | 59.967 | 60.038 | End: 3533 |
| Size | 20 | 20 | 209 |
(TC)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGAATCCAAGGCAGCCAGA | CCCACACAACAACATGGCAG | Start: 350 |
| Tm | 60.035 | 59.968 | End: 477 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
|---|
| Sequence | AATGAAGGTTTTGCGTGGCC | TCGTCGCTTGGGTTTGATGA | Start: 2377 |
| Tm | 59.967 | 59.966 | End: 2579 |
| Size | 20 | 20 | 203 |
| Primer 3 details |
|---|
| Sequence | TCATCAAACCCAAGCGACGA | CGGAACAGGGAGAAAGCCAT | Start: 2560 |
| Tm | 59.966 | 60.035 | End: 2720 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | GAGTGTGCATCCGGGTAACA | GGCCACGCAAAACCTTCATT | Start: 2266 |
| Tm | 60.037 | 59.967 | End: 2396 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | CCACCGTGTTTCCGAGAGAA | CGTCTCCCTCGTGAAGCTTT | Start: 3325 |
| Tm | 59.967 | 60.038 | End: 3533 |
| Size | 20 | 20 | 209 |
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