ssr_primer
Primer Details for TRINITY_DN12386_c1_g2_i8 |
|---|
| (ATT)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGGTCTTCGTAACCGTGG | AGGGTTCCCACAAAACTCCC | Start: 143 |
| Tm | 59.966 | 59.812 | End: 300 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
|---|
| Sequence | GCAGGATCTGTGGCATGGTA | GGGACAGGACCGTCTGAATC | Start: 2684 |
| Tm | 59.819 | 59.824 | End: 2941 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | GCAGGATCTGTGGCATGGTA | GGACAGGACCGTCTGAATCG | Start: 2684 |
| Tm | 59.819 | 60.179 | End: 2940 |
| Size | 20 | 20 | 257 |
| Primer 4 details |
|---|
| Sequence | TCGTGATCTTCTCTTGGCGG | AGGGTTCCCACAAAACTCCC | Start: 119 |
| Tm | 59.827 | 59.812 | End: 300 |
| Size | 20 | 20 | 182 |
| Primer 5 details |
|---|
| Sequence | CCCCAGCCGTTTTAGTCACA | ACAGCACTGCCCAAATCAGA | Start: 1515 |
| Tm | 60.251 | 59.889 | End: 1666 |
| Size | 20 | 20 | 152 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGGTCTTCGTAACCGTGG | AGGGTTCCCACAAAACTCCC | Start: 143 |
| Tm | 59.966 | 59.812 | End: 300 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
|---|
| Sequence | GCAGGATCTGTGGCATGGTA | GGGACAGGACCGTCTGAATC | Start: 2684 |
| Tm | 59.819 | 59.824 | End: 2941 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | GCAGGATCTGTGGCATGGTA | GGACAGGACCGTCTGAATCG | Start: 2684 |
| Tm | 59.819 | 60.179 | End: 2940 |
| Size | 20 | 20 | 257 |
| Primer 4 details |
|---|
| Sequence | TCGTGATCTTCTCTTGGCGG | AGGGTTCCCACAAAACTCCC | Start: 119 |
| Tm | 59.827 | 59.812 | End: 300 |
| Size | 20 | 20 | 182 |
| Primer 5 details |
|---|
| Sequence | CCCCAGCCGTTTTAGTCACA | ACAGCACTGCCCAAATCAGA | Start: 1515 |
| Tm | 60.251 | 59.889 | End: 1666 |
| Size | 20 | 20 | 152 |
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