ssr_primer
Primer Details for TRINITY_DN12384_c1_g3_i14 |
|---|
| (T)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAGAGTGCAGATGTGGC | AACACATCTGCGGTACACGT | Start: 989 |
| Tm | 60.036 | 59.967 | End: 1156 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | ACGTGTACCGCAGATGTGTT | CTGCTTCACGTTGGGAGGAT | Start: 1137 |
| Tm | 59.967 | 60.036 | End: 1397 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | TGCAAGAACCGCTCGAGATT | AGGGCCGTCAAAAGGATCAG | Start: 3947 |
| Tm | 60.037 | 60.035 | End: 4148 |
| Size | 20 | 20 | 202 |
| Primer 4 details |
|---|
| Sequence | AGGAAGAGTGCAGATGTGGC | ACGTATTGCGCCTTCCTTCA | Start: 989 |
| Tm | 60.036 | 60.037 | End: 1140 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | TGCAAGAACCGCTCGAGATT | TCAGGGCCGTCAAAAGGATC | Start: 3947 |
| Tm | 60.037 | 60.035 | End: 4150 |
| Size | 20 | 20 | 204 |
(A)11cataattatgatgcagccaacagctct(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAGAGTGCAGATGTGGC | AACACATCTGCGGTACACGT | Start: 989 |
| Tm | 60.036 | 59.967 | End: 1156 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | ACGTGTACCGCAGATGTGTT | CTGCTTCACGTTGGGAGGAT | Start: 1137 |
| Tm | 59.967 | 60.036 | End: 1397 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | TGCAAGAACCGCTCGAGATT | AGGGCCGTCAAAAGGATCAG | Start: 3947 |
| Tm | 60.037 | 60.035 | End: 4148 |
| Size | 20 | 20 | 202 |
| Primer 4 details |
|---|
| Sequence | AGGAAGAGTGCAGATGTGGC | ACGTATTGCGCCTTCCTTCA | Start: 989 |
| Tm | 60.036 | 60.037 | End: 1140 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | TGCAAGAACCGCTCGAGATT | TCAGGGCCGTCAAAAGGATC | Start: 3947 |
| Tm | 60.037 | 60.035 | End: 4150 |
| Size | 20 | 20 | 204 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAGAGTGCAGATGTGGC | AACACATCTGCGGTACACGT | Start: 989 |
| Tm | 60.036 | 59.967 | End: 1156 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | ACGTGTACCGCAGATGTGTT | CTGCTTCACGTTGGGAGGAT | Start: 1137 |
| Tm | 59.967 | 60.036 | End: 1397 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | TGCAAGAACCGCTCGAGATT | AGGGCCGTCAAAAGGATCAG | Start: 3947 |
| Tm | 60.037 | 60.035 | End: 4148 |
| Size | 20 | 20 | 202 |
| Primer 4 details |
|---|
| Sequence | AGGAAGAGTGCAGATGTGGC | ACGTATTGCGCCTTCCTTCA | Start: 989 |
| Tm | 60.036 | 60.037 | End: 1140 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | TGCAAGAACCGCTCGAGATT | TCAGGGCCGTCAAAAGGATC | Start: 3947 |
| Tm | 60.037 | 60.035 | End: 4150 |
| Size | 20 | 20 | 204 |
(GAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAGAGTGCAGATGTGGC | AACACATCTGCGGTACACGT | Start: 989 |
| Tm | 60.036 | 59.967 | End: 1156 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | ACGTGTACCGCAGATGTGTT | CTGCTTCACGTTGGGAGGAT | Start: 1137 |
| Tm | 59.967 | 60.036 | End: 1397 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | TGCAAGAACCGCTCGAGATT | AGGGCCGTCAAAAGGATCAG | Start: 3947 |
| Tm | 60.037 | 60.035 | End: 4148 |
| Size | 20 | 20 | 202 |
| Primer 4 details |
|---|
| Sequence | AGGAAGAGTGCAGATGTGGC | ACGTATTGCGCCTTCCTTCA | Start: 989 |
| Tm | 60.036 | 60.037 | End: 1140 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | TGCAAGAACCGCTCGAGATT | TCAGGGCCGTCAAAAGGATC | Start: 3947 |
| Tm | 60.037 | 60.035 | End: 4150 |
| Size | 20 | 20 | 204 |
(GA)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAGAGTGCAGATGTGGC | AACACATCTGCGGTACACGT | Start: 989 |
| Tm | 60.036 | 59.967 | End: 1156 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | ACGTGTACCGCAGATGTGTT | CTGCTTCACGTTGGGAGGAT | Start: 1137 |
| Tm | 59.967 | 60.036 | End: 1397 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | TGCAAGAACCGCTCGAGATT | AGGGCCGTCAAAAGGATCAG | Start: 3947 |
| Tm | 60.037 | 60.035 | End: 4148 |
| Size | 20 | 20 | 202 |
| Primer 4 details |
|---|
| Sequence | AGGAAGAGTGCAGATGTGGC | ACGTATTGCGCCTTCCTTCA | Start: 989 |
| Tm | 60.036 | 60.037 | End: 1140 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | TGCAAGAACCGCTCGAGATT | TCAGGGCCGTCAAAAGGATC | Start: 3947 |
| Tm | 60.037 | 60.035 | End: 4150 |
| Size | 20 | 20 | 204 |
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