Primer Details for TRINITY_DN12383_c0_g12_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTCCCGAACCCATGCTTT | ACTTGCTCCATCTTACACTACTGT | Start: 17 |
| Tm | 58.063 | 59.471 | End: 230 |
| Size | 20 | 24 | 214 |
| Primer 2 details | |||
| Sequence | AATTCCCGAACCCATGCTTT | TGAAACTTGCTCCATCTTACACT | Start: 17 |
| Tm | 58.063 | 58.027 | End: 234 |
| Size | 20 | 23 | 218 |
| Primer 3 details | |||
| Sequence | AATTCCCGAACCCATGCTTT | AACTTGCTCCATCTTACACTACTGT | Start: 17 |
| Tm | 58.063 | 59.988 | End: 231 |
| Size | 20 | 25 | 215 |
| Primer 4 details | |||
| Sequence | AATTCCCGAACCCATGCTTT | CTTGCTCCATCTTACACTACTGT | Start: 17 |
| Tm | 58.063 | 57.922 | End: 229 |
| Size | 20 | 23 | 213 |
| Primer 5 details | |||
| Sequence | AATTCCCGAACCCATGCTTT | ACTTGCTCCATCTTACACTACTG | Start: 17 |
| Tm | 58.063 | 57.922 | End: 230 |
| Size | 20 | 23 | 214 |
