ssr_primer
Primer Details for TRINITY_DN12379_c2_g5_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGCAAACTGGCATCTCCC | TTTTGCTTGGCACGCCTTAC | Start: 376 |
| Tm | 60.035 | 59.969 | End: 525 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
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| Sequence | GTAAGGCGTGCCAAGCAAAA | GGGAGACGGCCATCTTCATT | Start: 506 |
| Tm | 59.969 | 59.819 | End: 678 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
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| Sequence | GTAAGGCGTGCCAAGCAAAA | ATTGGGAGACGGCCATCTTC | Start: 506 |
| Tm | 59.969 | 59.819 | End: 681 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
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| Sequence | TGGCACAAAACACCCGAAAC | GACCTCCCTCCTGCCATTTC | Start: 1646 |
| Tm | 59.826 | 60.107 | End: 1906 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
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| Sequence | AGAGCAAACTGGCATCTCCC | TTGGGTAGCCCTTGCACTTC | Start: 376 |
| Tm | 60.035 | 60.251 | End: 587 |
| Size | 20 | 20 | 212 |
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