ssr_primer
Primer Details for TRINITY_DN12375_c0_g9_i1 |
|---|
| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTCGTGCTTGTTCGTTTGT | GAGACGACTCTCACCCGAAC | Start: 479 |
| Tm | 59.974 | 59.831 | End: 586 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
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| Sequence | CACCCCCGTCATGTAGTGTC | CGAAAGCGGTCGAAATGTCC | Start: 460 |
| Tm | 60.108 | 59.905 | End: 563 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
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| Sequence | GATTATGGCACCCCCGTCAT | CGAAAGCGGTCGAAATGTCC | Start: 452 |
| Tm | 59.89 | 59.905 | End: 563 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
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| Sequence | GCCATTGCTCGTCTTGCTTT | ACAAACGAACAAGCACGACG | Start: 333 |
| Tm | 59.758 | 59.974 | End: 498 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
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| Sequence | ATTATGGCACCCCCGTCATG | CGAAAGCGGTCGAAATGTCC | Start: 453 |
| Tm | 60.179 | 59.905 | End: 563 |
| Size | 20 | 20 | 111 |
(GAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTCGTGCTTGTTCGTTTGT | GAGACGACTCTCACCCGAAC | Start: 479 |
| Tm | 59.974 | 59.831 | End: 586 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
|---|
| Sequence | CACCCCCGTCATGTAGTGTC | CGAAAGCGGTCGAAATGTCC | Start: 460 |
| Tm | 60.108 | 59.905 | End: 563 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | GATTATGGCACCCCCGTCAT | CGAAAGCGGTCGAAATGTCC | Start: 452 |
| Tm | 59.89 | 59.905 | End: 563 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
|---|
| Sequence | GCCATTGCTCGTCTTGCTTT | ACAAACGAACAAGCACGACG | Start: 333 |
| Tm | 59.758 | 59.974 | End: 498 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
|---|
| Sequence | ATTATGGCACCCCCGTCATG | CGAAAGCGGTCGAAATGTCC | Start: 453 |
| Tm | 60.179 | 59.905 | End: 563 |
| Size | 20 | 20 | 111 |
(AAG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTCGTGCTTGTTCGTTTGT | GAGACGACTCTCACCCGAAC | Start: 479 |
| Tm | 59.974 | 59.831 | End: 586 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
|---|
| Sequence | CACCCCCGTCATGTAGTGTC | CGAAAGCGGTCGAAATGTCC | Start: 460 |
| Tm | 60.108 | 59.905 | End: 563 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | GATTATGGCACCCCCGTCAT | CGAAAGCGGTCGAAATGTCC | Start: 452 |
| Tm | 59.89 | 59.905 | End: 563 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
|---|
| Sequence | GCCATTGCTCGTCTTGCTTT | ACAAACGAACAAGCACGACG | Start: 333 |
| Tm | 59.758 | 59.974 | End: 498 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
|---|
| Sequence | ATTATGGCACCCCCGTCATG | CGAAAGCGGTCGAAATGTCC | Start: 453 |
| Tm | 60.179 | 59.905 | End: 563 |
| Size | 20 | 20 | 111 |
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