ssr_primer
Primer Details for TRINITY_DN12375_c0_g1_i10 |
|---|
| (A)17(AAG)7*aaaaaagaaagaattgaaaattccaataacactttcttccctatcctgaattacacaaaatggctgtgaggaggag(A)10gaataataatgaaaattagggagaaatgggggagatgaataaatatgagaaatatgatgagatcgtcaatttctaaactcagcaaattaaattaaa(T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGGGTCCGACCACTAGGA | TGTGGAGCACTTCTCTGCAG | Start: 1052 |
| Tm | 59.884 | 59.966 | End: 1179 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
|---|
| Sequence | AAAGGGTCCGACCACTAGGA | TGTGCATTTCCTCCAACGGA | Start: 1052 |
| Tm | 59.884 | 59.89 | End: 1151 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | ACGTTCTTTTCCACCGGGTT | TCTGTCATGCTCCATCGCTG | Start: 367 |
| Tm | 60.107 | 60.179 | End: 604 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | TTGTCTAGCGGTTGGTAGCG | TCTGTCATGCTCCATCGCTG | Start: 337 |
| Tm | 60.109 | 60.179 | End: 604 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | AAAGGGTCCGACCACTAGGA | GGCTTTGTTGCGGCCATTAT | Start: 1052 |
| Tm | 59.884 | 59.824 | End: 1204 |
| Size | 20 | 20 | 153 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGGGTCCGACCACTAGGA | TGTGGAGCACTTCTCTGCAG | Start: 1052 |
| Tm | 59.884 | 59.966 | End: 1179 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
|---|
| Sequence | AAAGGGTCCGACCACTAGGA | TGTGCATTTCCTCCAACGGA | Start: 1052 |
| Tm | 59.884 | 59.89 | End: 1151 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | ACGTTCTTTTCCACCGGGTT | TCTGTCATGCTCCATCGCTG | Start: 367 |
| Tm | 60.107 | 60.179 | End: 604 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | TTGTCTAGCGGTTGGTAGCG | TCTGTCATGCTCCATCGCTG | Start: 337 |
| Tm | 60.109 | 60.179 | End: 604 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | AAAGGGTCCGACCACTAGGA | GGCTTTGTTGCGGCCATTAT | Start: 1052 |
| Tm | 59.884 | 59.824 | End: 1204 |
| Size | 20 | 20 | 153 |
(GA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGGGTCCGACCACTAGGA | TGTGGAGCACTTCTCTGCAG | Start: 1052 |
| Tm | 59.884 | 59.966 | End: 1179 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
|---|
| Sequence | AAAGGGTCCGACCACTAGGA | TGTGCATTTCCTCCAACGGA | Start: 1052 |
| Tm | 59.884 | 59.89 | End: 1151 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | ACGTTCTTTTCCACCGGGTT | TCTGTCATGCTCCATCGCTG | Start: 367 |
| Tm | 60.107 | 60.179 | End: 604 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | TTGTCTAGCGGTTGGTAGCG | TCTGTCATGCTCCATCGCTG | Start: 337 |
| Tm | 60.109 | 60.179 | End: 604 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | AAAGGGTCCGACCACTAGGA | GGCTTTGTTGCGGCCATTAT | Start: 1052 |
| Tm | 59.884 | 59.824 | End: 1204 |
| Size | 20 | 20 | 153 |
(TTTA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGGGTCCGACCACTAGGA | TGTGGAGCACTTCTCTGCAG | Start: 1052 |
| Tm | 59.884 | 59.966 | End: 1179 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
|---|
| Sequence | AAAGGGTCCGACCACTAGGA | TGTGCATTTCCTCCAACGGA | Start: 1052 |
| Tm | 59.884 | 59.89 | End: 1151 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | ACGTTCTTTTCCACCGGGTT | TCTGTCATGCTCCATCGCTG | Start: 367 |
| Tm | 60.107 | 60.179 | End: 604 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | TTGTCTAGCGGTTGGTAGCG | TCTGTCATGCTCCATCGCTG | Start: 337 |
| Tm | 60.109 | 60.179 | End: 604 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | AAAGGGTCCGACCACTAGGA | GGCTTTGTTGCGGCCATTAT | Start: 1052 |
| Tm | 59.884 | 59.824 | End: 1204 |
| Size | 20 | 20 | 153 |
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